Matches in SemOpenAlex for { <https://semopenalex.org/work/W220172477> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W220172477 abstract "Abstract : The development of biological detectors for the military and homeland defense 26 relies on the ability to test hardware with biological materials that can be produced reliably 27 and reproducibly. To ensure occupational safety during the development of new devices, 28 the production and testing with biological materials necessitates compromises that include 29 selection of a virulent strains and inactivation using various techniques. The materials and 30 methods selected for production and post-production treatment can greatly influence 31 detection outcomes using sensitive DNA or antibody based systems. The optimal situation 32 would be to reproducibly create biological testing materials that accurately simulate the 33 true pathogen yet carry minimal risk to the scientists and engineers developing the 34 biosensors. In this study, avirulent Bacillus anthracis spores were used to demonstrate the 35 varied outcomes that can be induced by density gradient purification and post-growth 36 inactivation using gamma irradiation. The responses using Quantitative Polymerase Chain 37 Reaction, Electrochemiluminescence, Enzyme-Linked Immunosorbent Assay, 38 Bicinchoninic Acid assay, and protein electrophoresis varied significantly with treatment. 39 Gradient purification removed proteins that interfered with monoclonal and polyclonal 40 antibodies- abilities to form immunocomplexes in Electrochemiluminescent and ELISA 41 assays. Irradiation had a varied effect on qPCR depending on the sample preparation 42 employed and produced a reduced response in Electrochemiluminescent and ELISA 43 assays. Varied results from enumeration techniques show the importance of measuring the 44 dead cellular material and cellular debris contribution to any sample. Production and 45 material treatments need to conform to a yet to be established guideline that can bring 46 uniformity to available biological agent reference materials." @default.
- W220172477 created "2016-06-24" @default.
- W220172477 creator A5015999273 @default.
- W220172477 creator A5052679280 @default.
- W220172477 creator A5061074212 @default.
- W220172477 creator A5068028501 @default.
- W220172477 creator A5070194299 @default.
- W220172477 creator A5072052548 @default.
- W220172477 creator A5072698835 @default.
- W220172477 creator A5075823708 @default.
- W220172477 creator A5076490753 @default.
- W220172477 creator A5080820483 @default.
- W220172477 date "2011-02-23" @default.
- W220172477 modified "2023-09-26" @default.
- W220172477 title "Measuring the Variability of Treated Bacillus Anthracis Delta Stern Spores" @default.
- W220172477 hasPublicationYear "2011" @default.
- W220172477 type Work @default.
- W220172477 sameAs 220172477 @default.
- W220172477 citedByCount "0" @default.
- W220172477 crossrefType "journal-article" @default.
- W220172477 hasAuthorship W220172477A5015999273 @default.
- W220172477 hasAuthorship W220172477A5052679280 @default.
- W220172477 hasAuthorship W220172477A5061074212 @default.
- W220172477 hasAuthorship W220172477A5068028501 @default.
- W220172477 hasAuthorship W220172477A5070194299 @default.
- W220172477 hasAuthorship W220172477A5072052548 @default.
- W220172477 hasAuthorship W220172477A5072698835 @default.
- W220172477 hasAuthorship W220172477A5075823708 @default.
- W220172477 hasAuthorship W220172477A5076490753 @default.
- W220172477 hasAuthorship W220172477A5080820483 @default.
- W220172477 hasConcept C116084860 @default.
- W220172477 hasConcept C145802702 @default.
- W220172477 hasConcept C174618031 @default.
- W220172477 hasConcept C185592680 @default.
- W220172477 hasConcept C2780239966 @default.
- W220172477 hasConcept C43617362 @default.
- W220172477 hasConcept C523546767 @default.
- W220172477 hasConcept C54355233 @default.
- W220172477 hasConcept C55493867 @default.
- W220172477 hasConcept C72673979 @default.
- W220172477 hasConcept C86803240 @default.
- W220172477 hasConcept C89423630 @default.
- W220172477 hasConceptScore W220172477C116084860 @default.
- W220172477 hasConceptScore W220172477C145802702 @default.
- W220172477 hasConceptScore W220172477C174618031 @default.
- W220172477 hasConceptScore W220172477C185592680 @default.
- W220172477 hasConceptScore W220172477C2780239966 @default.
- W220172477 hasConceptScore W220172477C43617362 @default.
- W220172477 hasConceptScore W220172477C523546767 @default.
- W220172477 hasConceptScore W220172477C54355233 @default.
- W220172477 hasConceptScore W220172477C55493867 @default.
- W220172477 hasConceptScore W220172477C72673979 @default.
- W220172477 hasConceptScore W220172477C86803240 @default.
- W220172477 hasConceptScore W220172477C89423630 @default.
- W220172477 hasLocation W2201724771 @default.
- W220172477 hasOpenAccess W220172477 @default.
- W220172477 hasPrimaryLocation W2201724771 @default.
- W220172477 hasRelatedWork W1180326851 @default.
- W220172477 hasRelatedWork W1963762596 @default.
- W220172477 hasRelatedWork W2030065635 @default.
- W220172477 hasRelatedWork W2032306507 @default.
- W220172477 hasRelatedWork W2046445737 @default.
- W220172477 hasRelatedWork W2048373278 @default.
- W220172477 hasRelatedWork W2055466844 @default.
- W220172477 hasRelatedWork W2067966246 @default.
- W220172477 hasRelatedWork W2069150939 @default.
- W220172477 hasRelatedWork W2122376214 @default.
- W220172477 hasRelatedWork W2166844069 @default.
- W220172477 hasRelatedWork W2613152541 @default.
- W220172477 hasRelatedWork W301499840 @default.
- W220172477 hasRelatedWork W1712810132 @default.
- W220172477 hasRelatedWork W2518177795 @default.
- W220172477 hasRelatedWork W2926980635 @default.
- W220172477 isParatext "false" @default.
- W220172477 isRetracted "false" @default.
- W220172477 magId "220172477" @default.
- W220172477 workType "article" @default.