Matches in SemOpenAlex for { <https://semopenalex.org/work/W2201888224> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2201888224 endingPage "288" @default.
- W2201888224 startingPage "273" @default.
- W2201888224 abstract "The tripeptide glutathione (GSH) in its reduced form is a critical intracellular antioxidant that is essential to many physiological and toxicological processes. The rate-limiting enzyme in intracellular synthesis of GSH is the peptide bond formation between free glutamate and cysteine, mediated via γ-glutamylcysteine (GC) synthetase (EC 6.3.2.2: GCS). GSH synthesis is completed by the addition of glycine to GC, mediated via glutathione synthetase (EC 6.3.2.3; GS). We have developed a rapid and sensitive assay to measure both of these enzymes in the soluble fractions of tissues. For GCS activity, cytosolic fractions of tissue are incubated with an excess of cysteine and glutamate, and the reaction is stopped by addition of sulfosalicylic acid. For GS, dialyzed cytosol is incubated with an excess of GC and glycine. The products of each reaction are derivatized with monobromobimane, and the fluorescent bimane derivatives of glutathione and GC are separated by reverse-phase, ion-pair high performance liquid ..." @default.
- W2201888224 created "2016-06-24" @default.
- W2201888224 creator A5004279208 @default.
- W2201888224 creator A5068585243 @default.
- W2201888224 creator A5085883907 @default.
- W2201888224 date "1991-01-01" @default.
- W2201888224 modified "2023-09-27" @default.
- W2201888224 title "Determination of γ-Glutamylcysteine Synthetase and Glutathione Synthetase Activity by HPLC" @default.
- W2201888224 cites W1489359842 @default.
- W2201888224 cites W1503187265 @default.
- W2201888224 cites W1522251864 @default.
- W2201888224 cites W1535345009 @default.
- W2201888224 cites W1557095374 @default.
- W2201888224 cites W1588826234 @default.
- W2201888224 cites W1598225123 @default.
- W2201888224 cites W183675800 @default.
- W2201888224 cites W1975899947 @default.
- W2201888224 cites W2008460252 @default.
- W2201888224 cites W2015401602 @default.
- W2201888224 cites W2022780584 @default.
- W2201888224 cites W2024621231 @default.
- W2201888224 cites W2037776985 @default.
- W2201888224 cites W2057204560 @default.
- W2201888224 cites W2086551009 @default.
- W2201888224 cites W2087070965 @default.
- W2201888224 cites W2161984516 @default.
- W2201888224 cites W2163536072 @default.
- W2201888224 cites W2414388329 @default.
- W2201888224 cites W979665266 @default.
- W2201888224 doi "https://doi.org/10.3109/15376519109050858" @default.
- W2201888224 hasPublicationYear "1991" @default.
- W2201888224 type Work @default.
- W2201888224 sameAs 2201888224 @default.
- W2201888224 citedByCount "22" @default.
- W2201888224 countsByYear W22018882242012 @default.
- W2201888224 crossrefType "journal-article" @default.
- W2201888224 hasAuthorship W2201888224A5004279208 @default.
- W2201888224 hasAuthorship W2201888224A5068585243 @default.
- W2201888224 hasAuthorship W2201888224A5085883907 @default.
- W2201888224 hasConcept C179998833 @default.
- W2201888224 hasConcept C181199279 @default.
- W2201888224 hasConcept C185592680 @default.
- W2201888224 hasConcept C2779344277 @default.
- W2201888224 hasConcept C43617362 @default.
- W2201888224 hasConcept C538909803 @default.
- W2201888224 hasConcept C55493867 @default.
- W2201888224 hasConceptScore W2201888224C179998833 @default.
- W2201888224 hasConceptScore W2201888224C181199279 @default.
- W2201888224 hasConceptScore W2201888224C185592680 @default.
- W2201888224 hasConceptScore W2201888224C2779344277 @default.
- W2201888224 hasConceptScore W2201888224C43617362 @default.
- W2201888224 hasConceptScore W2201888224C538909803 @default.
- W2201888224 hasConceptScore W2201888224C55493867 @default.
- W2201888224 hasIssue "4" @default.
- W2201888224 hasLocation W22018882241 @default.
- W2201888224 hasOpenAccess W2201888224 @default.
- W2201888224 hasPrimaryLocation W22018882241 @default.
- W2201888224 hasRelatedWork W1199623702 @default.
- W2201888224 hasRelatedWork W1263321344 @default.
- W2201888224 hasRelatedWork W162897110 @default.
- W2201888224 hasRelatedWork W2040969209 @default.
- W2201888224 hasRelatedWork W2063541543 @default.
- W2201888224 hasRelatedWork W2163536072 @default.
- W2201888224 hasRelatedWork W2171780956 @default.
- W2201888224 hasRelatedWork W2770167399 @default.
- W2201888224 hasRelatedWork W2775343545 @default.
- W2201888224 hasRelatedWork W2304798752 @default.
- W2201888224 hasVolume "1" @default.
- W2201888224 isParatext "false" @default.
- W2201888224 isRetracted "false" @default.
- W2201888224 magId "2201888224" @default.
- W2201888224 workType "article" @default.