FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2203294517> ?p ?o ?g. }

• W2203294517 endingPage "100" @default.
• W2203294517 startingPage "89" @default.
• W2203294517 abstract "Anesthetic contact residues in γ-aminobutyric acid type A (GABAA) receptors have been identified using photolabels, including two propofol derivatives. O-propofol diazirine labels H267 in β3 and α1β3 receptors, whereas m-azi-propofol labels other residues in intersubunit clefts of α1β3. Neither label has been studied in αβγ receptors, the most common isoform in mammalian brain. In αβγ receptors, other anesthetic derivatives photolabel m-azi-propofol-labeled residues, but not βH267. The authors' structural homology model of α1β3γ2L receptors suggests that β3H267 may abut some of these sites.Substituted cysteine modification-protection was used to test β3H267C interactions with four potent anesthetics: propofol, etomidate, alphaxalone, and R-5-allyl-1-methyl-5-(m-trifluoromethyl-diazirinylphenyl) barbituric acid (mTFD-MPAB). The authors expressed α1β3γ2L or α1β3H267Cγ2L GABAA receptors in Xenopus oocytes. The authors used voltage clamp electrophysiology to assess receptor sensitivity to γ-aminobutyric acid (GABA) and anesthetics and to compare p-chloromercuribenzenesulfonate modification rates with GABA versus GABA plus anesthetics.Enhancement of low GABA (eliciting 5% of maximum) responses by equihypnotic concentrations of all four anesthetics was similar in α1β3γ2L and α1β3H267Cγ2L receptors (n > 3). Direct activation of α1β3H267Cγ2L receptors, but not α1β3γ2L, by mTFD-MPAB and propofol was significantly greater than the other anesthetics. Modification of β3H267C by p-chloromercuribenzenesulfonate (n > 4) was rapid and accelerated by GABA. Only mTFD-MPAB slowed β3H267C modification (approximately twofold; P = 0.011).β3H267 in α1β3γ2L GABAA receptors contacts mTFD-MPAB, but not propofol. The study results suggest that β3H267 is near the periphery of one or both transmembrane intersubunit (α+/β- and γ+/β-) pockets where both mTFD-MPAB and propofol bind." @default.
• W2203294517 created "2016-06-24" @default.
• W2203294517 creator A5034002459 @default.
• W2203294517 creator A5050184660 @default.
• W2203294517 date "2016-01-01" @default.
• W2203294517 modified "2023-10-16" @default.
• W2203294517 title "A Cysteine Substitution Probes β3H267 Interactions with Propofol and Other Potent Anesthetics in α1β3γ2L γ-Aminobutyric Acid Type A Receptors" @default.
• W2203294517 cites W1482497049 @default.
• W2203294517 cites W1592442520 @default.
• W2203294517 cites W1668093331 @default.
• W2203294517 cites W1830975445 @default.
• W2203294517 cites W1972473633 @default.
• W2203294517 cites W1972584910 @default.
• W2203294517 cites W1975239375 @default.
• W2203294517 cites W1982371070 @default.
• W2203294517 cites W1983756509 @default.
• W2203294517 cites W1987073960 @default.
• W2203294517 cites W1988562852 @default.
• W2203294517 cites W2003468829 @default.
• W2203294517 cites W2004467259 @default.
• W2203294517 cites W2011785924 @default.
• W2203294517 cites W2017227716 @default.
• W2203294517 cites W2017264248 @default.
• W2203294517 cites W2019622450 @default.
• W2203294517 cites W2027356603 @default.
• W2203294517 cites W2028046413 @default.
• W2203294517 cites W2030604934 @default.
• W2203294517 cites W2047478873 @default.
• W2203294517 cites W2053311055 @default.
• W2203294517 cites W2054373571 @default.
• W2203294517 cites W2054534931 @default.
• W2203294517 cites W2058571413 @default.
• W2203294517 cites W2063157730 @default.
• W2203294517 cites W2069566746 @default.
• W2203294517 cites W2074287295 @default.
• W2203294517 cites W2085913208 @default.
• W2203294517 cites W2087583001 @default.
• W2203294517 cites W2091486815 @default.
• W2203294517 cites W2100231995 @default.
• W2203294517 cites W2123528614 @default.
• W2203294517 cites W2131961672 @default.
• W2203294517 cites W2135390742 @default.
• W2203294517 cites W2145922165 @default.
• W2203294517 cites W2145980353 @default.
• W2203294517 cites W2149362408 @default.
• W2203294517 cites W2150456903 @default.
• W2203294517 cites W2154251024 @default.
• W2203294517 cites W2161329715 @default.
• W2203294517 cites W2161355408 @default.
• W2203294517 cites W2170087378 @default.
• W2203294517 cites W4324013584 @default.
• W2203294517 doi "https://doi.org/10.1097/aln.0000000000000934" @default.
• W2203294517 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4681607" @default.
• W2203294517 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26569173" @default.
• W2203294517 hasPublicationYear "2016" @default.
• W2203294517 type Work @default.
• W2203294517 sameAs 2203294517 @default.
• W2203294517 citedByCount "11" @default.
• W2203294517 countsByYear W22032945172016 @default.
• W2203294517 countsByYear W22032945172018 @default.
• W2203294517 countsByYear W22032945172019 @default.
• W2203294517 countsByYear W22032945172021 @default.
• W2203294517 countsByYear W22032945172022 @default.
• W2203294517 crossrefType "journal-article" @default.
• W2203294517 hasAuthorship W2203294517A5034002459 @default.
• W2203294517 hasAuthorship W2203294517A5050184660 @default.
• W2203294517 hasBestOaLocation W22032945172 @default.
• W2203294517 hasConcept C168258287 @default.
• W2203294517 hasConcept C170493617 @default.
• W2203294517 hasConcept C185592680 @default.
• W2203294517 hasConcept C19876734 @default.
• W2203294517 hasConcept C203270574 @default.
• W2203294517 hasConcept C26702167 @default.
• W2203294517 hasConcept C2776277131 @default.
• W2203294517 hasConcept C2778162923 @default.
• W2203294517 hasConcept C2778938600 @default.
• W2203294517 hasConcept C2779414652 @default.
• W2203294517 hasConcept C2779570518 @default.
• W2203294517 hasConcept C2780763569 @default.
• W2203294517 hasConcept C42219234 @default.
• W2203294517 hasConcept C55493867 @default.
• W2203294517 hasConcept C71240020 @default.
• W2203294517 hasConcept C71924100 @default.
• W2203294517 hasConcept C79636183 @default.
• W2203294517 hasConcept C80161118 @default.
• W2203294517 hasConcept C9224880 @default.
• W2203294517 hasConcept C98274493 @default.
• W2203294517 hasConceptScore W2203294517C168258287 @default.
• W2203294517 hasConceptScore W2203294517C170493617 @default.
• W2203294517 hasConceptScore W2203294517C185592680 @default.
• W2203294517 hasConceptScore W2203294517C19876734 @default.
• W2203294517 hasConceptScore W2203294517C203270574 @default.
• W2203294517 hasConceptScore W2203294517C26702167 @default.
• W2203294517 hasConceptScore W2203294517C2776277131 @default.
• W2203294517 hasConceptScore W2203294517C2778162923 @default.
• W2203294517 hasConceptScore W2203294517C2778938600 @default.
• W2203294517 hasConceptScore W2203294517C2779414652 @default.
• W2203294517 hasConceptScore W2203294517C2779570518 @default.

• next