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- W2203372068 abstract "Microsatellite markers are valuable tools for determining amount and distribution of genetic diversity and differentiation within and between populations. In this study we examined the level of microsatellite variability within and among five populations of Lobelia villosa, a rare Hawaiian endemic lobeliad on the island of Kaua‘i. Populations of L. villosa were sampled from two regions on Kaua‘i: two populations at the Kilohana Lookout area and three populations from Alaka‘i Swamp. Nineteen microsatellite DNA primers were developed for L. villosa, 12 of which demonstrated polymorphism and were subsequently multiplexed and labeled for genotyping. An overall moderate degree of genetic differentiation was found within and between populations. Pairwise FST data showed population structure, and analysis with Structure software indicated two genetic clusters (K = 2) corresponding to the two sampled geographic regions. Although L. villosa exhibits moderate diversity, which exceeds that of other Hawaiian endemics with restricted distributions, measurements of FIS were positive across 10 out of 12 loci, suggesting that inbreeding is occurring at the population level." @default.
- W2203372068 created "2016-06-24" @default.
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- W2203372068 date "2015-07-01" @default.
- W2203372068 modified "2023-10-16" @default.
- W2203372068 title "Genetic Structure and Diversity of a Rare Hawaiian Endemic,Lobelia villosa(Campanulaceae: Lobelioideae)" @default.
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- W2203372068 doi "https://doi.org/10.2984/69.3.5" @default.
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