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- W220352711 abstract "The banana (Musa spp., AAA) genome is continuously expanding due to high frequency of somaclonal variation. Because of this increasing diversity, identification and taxonomic classification of banana cultivars have become difficult and often subject to disagreement. In this paper, we describe the development of an auxiliary protocol in converting negative to positive RAPD markers that can discriminate closely related Musa, AAA cultivars. Using arbitrary primer A13, we identified negative RAPD markers A133.0 and A131.3 specifically for S4 (selection from Giant Cavendish subgroup, AAA) and S8 (‘Morado’ from ‘Red’ and ‘Green Red’ subgroup, AAA), respectively. Fragments corresponding to these missing bands were sequenced and used as templates to design new primers targeted around the 3’end of the original primer-annealing site. Two of these primers, Ba3.0A and Ba1.3A, successfully generated positive markers consistently amplified as Ba3.0A0.8 and Ba1.3A0.6 for S4 and S8, respectively. Since the resulting positive markers can be sequenced and longer primer pairs can be designed, this marker-like SCAR can become highly reproducible to discriminate S4 and S8 from among genetically related and morphologically indistinguishable Musa, AAA cultivars. The results of the test demonstrate the value of an auxiliary tool that can be used in any RAPD-based marker system using various DNA sources. In addition, this provides an alternative method to screening more arbitrary primers in generating positive markers especially in situations when negative RAPD markers have been already identified." @default.
- W220352711 created "2016-06-24" @default.
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- W220352711 date "2003-01-01" @default.
- W220352711 modified "2023-09-23" @default.
- W220352711 title "Conversion of negative to positive DNA markers: development of auxiliary tool for a RAPD-based marker system in banana (Musa spp., AAA)." @default.
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