FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2204078315> ?p ?o ?g. }

• W2204078315 endingPage "218" @default.
• W2204078315 startingPage "209" @default.
• W2204078315 abstract "Erysipelothrix rhusiopathiae is a Gram-positive bacterium pathogenic to many species of birds and mammals, including humans. The main feature of its peptidoglycan is the presence of l-alanine at position 3 of the peptide stem. In the present work, we cloned the murE gene from E. rhusiopathiae and purified the corresponding protein as His6-tagged form. Enzymatic assays showed that E. rhusiopathiae MurE was indeed an l-alanine-adding enzyme. Surprisingly, it was also able, although to a lesser extent, to add meso-diaminopimelic acid, the amino acid found at position 3 in many Gram-negative bacteria, Bacilli and Mycobacteria. Sequence alignment of MurE enzymes from E. rhusiopathiae and Escherichia coli revealed that the DNPR motif that is characteristic of meso-diaminopimelate-adding enzymes was replaced by HDNR. The role of the latter motif in the interaction with l-alanine and meso-diaminopimelic acid was demonstrated by site-directed mutagenesis experiments and the construction of a homology model. The overexpression of the E. rhusiopathiae murE gene in E. coli resulted in the incorporation of l-alanine at position 3 of the peptide part of peptidoglycan." @default.
• W2204078315 created "2016-06-24" @default.
• W2204078315 creator A5013720618 @default.
• W2204078315 creator A5048552165 @default.
• W2204078315 creator A5058899747 @default.
• W2204078315 creator A5067176307 @default.
• W2204078315 creator A5068216286 @default.
• W2204078315 creator A5068592665 @default.
• W2204078315 creator A5075349013 @default.
• W2204078315 creator A5082261374 @default.
• W2204078315 date "2016-02-01" @default.
• W2204078315 modified "2023-10-17" @default.
• W2204078315 title "Unusual substrate specificity of the peptidoglycan MurE ligase from Erysipelothrix rhusiopathiae" @default.
• W2204078315 cites W1513723427 @default.
• W2204078315 cites W1526556374 @default.
• W2204078315 cites W1723438040 @default.
• W2204078315 cites W1836108890 @default.
• W2204078315 cites W1837763899 @default.
• W2204078315 cites W1892425163 @default.
• W2204078315 cites W1964854182 @default.
• W2204078315 cites W1969972591 @default.
• W2204078315 cites W1973945520 @default.
• W2204078315 cites W1980101822 @default.
• W2204078315 cites W1991486518 @default.
• W2204078315 cites W1994909642 @default.
• W2204078315 cites W2013045696 @default.
• W2204078315 cites W2020350008 @default.
• W2204078315 cites W2029141196 @default.
• W2204078315 cites W2040215775 @default.
• W2204078315 cites W2050584128 @default.
• W2204078315 cites W2052779789 @default.
• W2204078315 cites W2061778046 @default.
• W2204078315 cites W2079494225 @default.
• W2204078315 cites W2100590703 @default.
• W2204078315 cites W2110492858 @default.
• W2204078315 cites W2114437078 @default.
• W2204078315 cites W2114520383 @default.
• W2204078315 cites W2128123492 @default.
• W2204078315 cites W2137015675 @default.
• W2204078315 cites W2139130660 @default.
• W2204078315 cites W2147044107 @default.
• W2204078315 cites W2148325233 @default.
• W2204078315 cites W2159104819 @default.
• W2204078315 cites W2163435856 @default.
• W2204078315 cites W4230502249 @default.
• W2204078315 cites W4252700310 @default.
• W2204078315 doi "https://doi.org/10.1016/j.biochi.2015.12.006" @default.
• W2204078315 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26700151" @default.
• W2204078315 hasPublicationYear "2016" @default.
• W2204078315 type Work @default.
• W2204078315 sameAs 2204078315 @default.
• W2204078315 citedByCount "5" @default.
• W2204078315 countsByYear W22040783152018 @default.
• W2204078315 countsByYear W22040783152019 @default.
• W2204078315 countsByYear W22040783152020 @default.
• W2204078315 countsByYear W22040783152022 @default.
• W2204078315 crossrefType "journal-article" @default.
• W2204078315 hasAuthorship W2204078315A5013720618 @default.
• W2204078315 hasAuthorship W2204078315A5048552165 @default.
• W2204078315 hasAuthorship W2204078315A5058899747 @default.
• W2204078315 hasAuthorship W2204078315A5067176307 @default.
• W2204078315 hasAuthorship W2204078315A5068216286 @default.
• W2204078315 hasAuthorship W2204078315A5068592665 @default.
• W2204078315 hasAuthorship W2204078315A5075349013 @default.
• W2204078315 hasAuthorship W2204078315A5082261374 @default.
• W2204078315 hasConcept C104317684 @default.
• W2204078315 hasConcept C143065580 @default.
• W2204078315 hasConcept C16318435 @default.
• W2204078315 hasConcept C167625842 @default.
• W2204078315 hasConcept C181199279 @default.
• W2204078315 hasConcept C2776634448 @default.
• W2204078315 hasConcept C2778255925 @default.
• W2204078315 hasConcept C2779425345 @default.
• W2204078315 hasConcept C2779856020 @default.
• W2204078315 hasConcept C2780227090 @default.
• W2204078315 hasConcept C515207424 @default.
• W2204078315 hasConcept C547475151 @default.
• W2204078315 hasConcept C55493867 @default.
• W2204078315 hasConcept C86803240 @default.
• W2204078315 hasConcept C89423630 @default.
• W2204078315 hasConceptScore W2204078315C104317684 @default.
• W2204078315 hasConceptScore W2204078315C143065580 @default.
• W2204078315 hasConceptScore W2204078315C16318435 @default.
• W2204078315 hasConceptScore W2204078315C167625842 @default.
• W2204078315 hasConceptScore W2204078315C181199279 @default.
• W2204078315 hasConceptScore W2204078315C2776634448 @default.
• W2204078315 hasConceptScore W2204078315C2778255925 @default.
• W2204078315 hasConceptScore W2204078315C2779425345 @default.
• W2204078315 hasConceptScore W2204078315C2779856020 @default.
• W2204078315 hasConceptScore W2204078315C2780227090 @default.
• W2204078315 hasConceptScore W2204078315C515207424 @default.
• W2204078315 hasConceptScore W2204078315C547475151 @default.
• W2204078315 hasConceptScore W2204078315C55493867 @default.
• W2204078315 hasConceptScore W2204078315C86803240 @default.
• W2204078315 hasConceptScore W2204078315C89423630 @default.
• W2204078315 hasFunder F4320322554 @default.
• W2204078315 hasFunder F4320322892 @default.
• W2204078315 hasLocation W22040783151 @default.

• next