FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2204384758> ?p ?o ?g. }

• W2204384758 abstract "Investigations of how ligands specifically bind to dopamine receptors and how receptorinteracting proteins associate with dopamine receptors are important to the development of novel therapeutic strategies and the understanding of function of the central nervous system. The goals of my dissertation are to identify the structural determinants of pharmacological specificity between D1 and D2 dopamine receptors and to identify the arrestin-binding sites of D2 and D3 dopamine receptors.* To achieve the first goal, I hypothesized that pharmacological differentiation between D1 and D2 dopamine receptors results from interactions of selective ligands with nonconserved residues lining the binding pocket. Amino acid residues in the D2 receptor were mutated to the corresponding aligned residues in the D1 receptor and vice versa and the receptors were expressed in human embryonic kidney (HEK) 293 cells. The affinities of the mutant receptors for D 1and Dz-selective antagonists were determined by radio ligand binding, followed by receptor homology modeling and ligand docking. I identified not only two residues that contribute to differential ligand binding profiles by interacting directly with ligands, but also one residue that affects D1/D2 pharmacological selectivity presumably by affecting the overall shape of binding pocket. To investigate the arrestin-binding sites of D2 and D3 dopamine receptors, first, the second and third intracellular loops (IC2 and IC3) ofboth receptor subtypes were examined for their direct interactions with purified arrestin-2 and-3 using glutathinone-S-transferase (GST) pull-down assays. Secondly, chimeric, truncation, and substitution mutants were constructed to test their ability to bind purified arrestin-3. Data from these studies indicate that higher binding affinity of arrestin-3 to D2 receptor over D3 * D1, D2, D3, D4 and D5 with subscripted numerals, genetic subtypes Dl or Dl-like and D2 or D2-like, pharmacological subtypes" @default.
• W2204384758 created "2016-06-24" @default.
• W2204384758 creator A5031286677 @default.
• W2204384758 date "2006-01-01" @default.
• W2204384758 modified "2023-09-23" @default.
• W2204384758 title "Structure and function of dopamine receptors : ligand binding and arrestin binding" @default.
• W2204384758 cites W10039931 @default.
• W2204384758 cites W130778363 @default.
• W2204384758 cites W1508211596 @default.
• W2204384758 cites W1520765192 @default.
• W2204384758 cites W1520788671 @default.
• W2204384758 cites W1529918245 @default.
• W2204384758 cites W1547923480 @default.
• W2204384758 cites W1562624919 @default.
• W2204384758 cites W1581533309 @default.
• W2204384758 cites W1604208828 @default.
• W2204384758 cites W1654237604 @default.
• W2204384758 cites W1689798268 @default.
• W2204384758 cites W1805958564 @default.
• W2204384758 cites W1895903557 @default.
• W2204384758 cites W1927946021 @default.
• W2204384758 cites W1945187946 @default.
• W2204384758 cites W1966034205 @default.
• W2204384758 cites W1966092598 @default.
• W2204384758 cites W1966627438 @default.
• W2204384758 cites W1966985448 @default.
• W2204384758 cites W1969041583 @default.
• W2204384758 cites W1969913785 @default.
• W2204384758 cites W1971417227 @default.
• W2204384758 cites W1973334992 @default.
• W2204384758 cites W1975809719 @default.
• W2204384758 cites W1977234970 @default.
• W2204384758 cites W1977533543 @default.
• W2204384758 cites W1977951315 @default.
• W2204384758 cites W1979815209 @default.
• W2204384758 cites W1980686307 @default.
• W2204384758 cites W1981994593 @default.
• W2204384758 cites W1983177275 @default.
• W2204384758 cites W1985582613 @default.
• W2204384758 cites W1985624576 @default.
• W2204384758 cites W1986144006 @default.
• W2204384758 cites W1986478158 @default.
• W2204384758 cites W1987672004 @default.
• W2204384758 cites W1992334846 @default.
• W2204384758 cites W1993982282 @default.
• W2204384758 cites W1994941602 @default.
• W2204384758 cites W1997085676 @default.
• W2204384758 cites W2000949690 @default.
• W2204384758 cites W2001406669 @default.
• W2204384758 cites W2001566515 @default.
• W2204384758 cites W2003832177 @default.
• W2204384758 cites W2004755511 @default.
• W2204384758 cites W2007227244 @default.
• W2204384758 cites W2009778458 @default.
• W2204384758 cites W2010512930 @default.
• W2204384758 cites W2010556242 @default.
• W2204384758 cites W2011067926 @default.
• W2204384758 cites W2012070600 @default.
• W2204384758 cites W2012571494 @default.
• W2204384758 cites W2013190001 @default.
• W2204384758 cites W2013851440 @default.
• W2204384758 cites W2016630919 @default.
• W2204384758 cites W2017549779 @default.
• W2204384758 cites W2017875329 @default.
• W2204384758 cites W2021100900 @default.
• W2204384758 cites W2021388743 @default.
• W2204384758 cites W2022576341 @default.
• W2204384758 cites W2023132550 @default.
• W2204384758 cites W2023661096 @default.
• W2204384758 cites W2026180033 @default.
• W2204384758 cites W2028466783 @default.
• W2204384758 cites W2030903240 @default.
• W2204384758 cites W2030905853 @default.
• W2204384758 cites W2031422121 @default.
• W2204384758 cites W2032243789 @default.
• W2204384758 cites W2033688302 @default.
• W2204384758 cites W2038493502 @default.
• W2204384758 cites W2040442394 @default.
• W2204384758 cites W2042170152 @default.
• W2204384758 cites W2047110318 @default.
• W2204384758 cites W2047619967 @default.
• W2204384758 cites W2047930419 @default.
• W2204384758 cites W2048669565 @default.
• W2204384758 cites W2048715086 @default.
• W2204384758 cites W2050218326 @default.
• W2204384758 cites W2052297575 @default.
• W2204384758 cites W2057136443 @default.
• W2204384758 cites W2058396154 @default.
• W2204384758 cites W2059220343 @default.
• W2204384758 cites W2059230444 @default.
• W2204384758 cites W2059536365 @default.
• W2204384758 cites W2065241932 @default.
• W2204384758 cites W2065295009 @default.
• W2204384758 cites W2067838356 @default.
• W2204384758 cites W2071784044 @default.
• W2204384758 cites W2072096730 @default.
• W2204384758 cites W2074309655 @default.
• W2204384758 cites W2074631079 @default.
• W2204384758 cites W2075796682 @default.
• W2204384758 cites W2079463706 @default.

• next