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- W2204663353 abstract "Monospecific antibodies were used to screen a protein fusion clone bank containing small chromosomal fragments of the wild type Pseudomonas syringae pv. syringae isolate R 32 DNA. A positively reacting clone was used to insert a kanamycin cassette and was marker exchanged into the wild type strain R 32. A successful inactivation of the gene coding for the 31 kDa protein could be demonstrated by the lack of the monospecific antibody reaction. This mutant caused attenuated symptoms on bean plants and a change in the HR on tobacco ieaves. A complementation of the marker exchanged mutant resulted in the serological detection of the 31 kDa protein but only a partial restoration of the symptoms on beans and the HR on tobacco. However, when this DNA fragment covering the coding region of the 31 kDa protein was used to hybridize with other related or unrelated pseudomonads, all tested bacteria showed a hybridization signal even though the monospecific antibodies showed a reaction only with the plant pathogenic pseudomonads. The applicability of the monospecific antibodies for diagnosis purpose is demonstrated for Pseudomonas syringae pv. phaseolicola." @default.
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- W2204663353 date "1997-01-01" @default.
- W2204663353 modified "2023-09-23" @default.
- W2204663353 title "The Potential Use of Monospecific Antibodies Against Pseudomonas syringae Pathovars to Elucidate Host Parasite Interactions" @default.
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- W2204663353 doi "https://doi.org/10.1007/978-94-011-5472-7_65" @default.
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