FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2205513353> ?p ?o ?g. }

• W2205513353 endingPage "985" @default.
• W2205513353 startingPage "978" @default.
• W2205513353 abstract "Interferon Regulatory Factors (IRFs) belong to a family of transcription factor involved in transcriptional regulation of type I IFN and IFN-stimulated genes (ISG) in cells. In the present study, an IRF3 full-length cDNA (termed CiIRF3, JX999055) and its promoter sequence were cloned by homology cloning strategy and genome walking from grass carp (Ctenopharyngodon idella). The full-length cDNA sequence of CiIRF3 is comprised of a 5'UTR (195 bp), a 3'UTR (269 bp) and a largest open reading frame (ORF) of 1377 bp encoding a polypeptide of 458 amino acids. CiIRF3 has a conservative DNA-binding domain (DBD) at N-terminal and a relatively conserved interferon regulatory factors association domain (IAD). Phylogenetic tree analysis indicated that CiIRF3 gathers together with other IRF-3 from higher vertebrates in the same branch. The promoter sequence of CiIRF3 (596 bp) consists of three IRF-E, a C/EBP beta, a NF-kappa B and a TATA-BOX. CiIRF3 was constitutively expressed at low level in different grass carp tissues but was rapidly up-regulated with Poly I:C stimulation. To study the molecular mechanism of CiIRF3 regulating the transcription of IRFs, CiIRF3 was expressed in Escherichia coli BL21 and purified by affinity chromatography with the Ni-NTA His-Bind Resin. Gel mobility shift assays revealed the affinity of CiIRF3 protein with promoters of CiIRF1, CiIRF2, CiIRF3 and CiIRF7 respectively. Then, CIK cells were co-transfected with pcDNA3.1-CiIRF3, pGL3-promotor (pGL3-CiIRF1, pGL3-CiIRF2, pGL3-CiIRF3, pGL3-CiIRF7) and luciferase reporter vector respectively. The cotransfection experiment showed that CiIRF3 increased the promoter activity of CiIRF1, CiIRF2, CiIRF3 and CiIRF7. Furthermore, overexpression of CiIRF3 in CIK cells also up-regulated the expressions of CiIRF1, CiIRF2, CiIRF3 and CiIRF7. So, CiIRF3 can improve the transcriptional level of CiIRF1, CiIRF2, CiIRF3 and CiIRF7." @default.
• W2205513353 created "2016-06-24" @default.
• W2205513353 creator A5002802549 @default.
• W2205513353 creator A5006012404 @default.
• W2205513353 creator A5014548453 @default.
• W2205513353 creator A5015574044 @default.
• W2205513353 creator A5028858211 @default.
• W2205513353 creator A5054208326 @default.
• W2205513353 creator A5054526577 @default.
• W2205513353 creator A5056487633 @default.
• W2205513353 creator A5084134381 @default.
• W2205513353 creator A5087899506 @default.
• W2205513353 creator A5089871946 @default.
• W2205513353 date "2015-12-01" @default.
• W2205513353 modified "2023-10-18" @default.
• W2205513353 title "Fish IRF3 up-regulates the transcriptional level of IRF1, IRF2, IRF3 and IRF7 in CIK cells" @default.
• W2205513353 cites W1555739603 @default.
• W2205513353 cites W1590945123 @default.
• W2205513353 cites W1962468650 @default.
• W2205513353 cites W1965342008 @default.
• W2205513353 cites W1967369695 @default.
• W2205513353 cites W1973701834 @default.
• W2205513353 cites W1974876709 @default.
• W2205513353 cites W1978990512 @default.
• W2205513353 cites W1979281412 @default.
• W2205513353 cites W1979667801 @default.
• W2205513353 cites W1983768032 @default.
• W2205513353 cites W1984632540 @default.
• W2205513353 cites W1990700589 @default.
• W2205513353 cites W1994620260 @default.
• W2205513353 cites W2000329335 @default.
• W2205513353 cites W2001653597 @default.
• W2205513353 cites W2010144425 @default.
• W2205513353 cites W2015851287 @default.
• W2205513353 cites W2016717901 @default.
• W2205513353 cites W2017784906 @default.
• W2205513353 cites W2025907872 @default.
• W2205513353 cites W2028233981 @default.
• W2205513353 cites W2028850807 @default.
• W2205513353 cites W2029052476 @default.
• W2205513353 cites W2029275361 @default.
• W2205513353 cites W2031489614 @default.
• W2205513353 cites W2039119595 @default.
• W2205513353 cites W2041309209 @default.
• W2205513353 cites W2054575463 @default.
• W2205513353 cites W2055545565 @default.
• W2205513353 cites W2056502408 @default.
• W2205513353 cites W2061881258 @default.
• W2205513353 cites W2073145822 @default.
• W2205513353 cites W2073633906 @default.
• W2205513353 cites W2077291760 @default.
• W2205513353 cites W2077819506 @default.
• W2205513353 cites W2079085384 @default.
• W2205513353 cites W2085614841 @default.
• W2205513353 cites W2097841705 @default.
• W2205513353 cites W2099371842 @default.
• W2205513353 cites W2099457187 @default.
• W2205513353 cites W2102650486 @default.
• W2205513353 cites W2115616959 @default.
• W2205513353 cites W2123026728 @default.
• W2205513353 cites W2130576390 @default.
• W2205513353 cites W2142368962 @default.
• W2205513353 cites W2147640266 @default.
• W2205513353 cites W2148154918 @default.
• W2205513353 cites W2155706758 @default.
• W2205513353 cites W2157280988 @default.
• W2205513353 cites W2157339124 @default.
• W2205513353 cites W2162154045 @default.
• W2205513353 cites W2170654373 @default.
• W2205513353 doi "https://doi.org/10.1016/j.fsi.2015.10.039" @default.
• W2205513353 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26545324" @default.
• W2205513353 hasPublicationYear "2015" @default.
• W2205513353 type Work @default.
• W2205513353 sameAs 2205513353 @default.
• W2205513353 citedByCount "13" @default.
• W2205513353 countsByYear W22055133532016 @default.
• W2205513353 countsByYear W22055133532017 @default.
• W2205513353 countsByYear W22055133532018 @default.
• W2205513353 countsByYear W22055133532019 @default.
• W2205513353 countsByYear W22055133532021 @default.
• W2205513353 countsByYear W22055133532023 @default.
• W2205513353 crossrefType "journal-article" @default.
• W2205513353 hasAuthorship W2205513353A5002802549 @default.
• W2205513353 hasAuthorship W2205513353A5006012404 @default.
• W2205513353 hasAuthorship W2205513353A5014548453 @default.
• W2205513353 hasAuthorship W2205513353A5015574044 @default.
• W2205513353 hasAuthorship W2205513353A5028858211 @default.
• W2205513353 hasAuthorship W2205513353A5054208326 @default.
• W2205513353 hasAuthorship W2205513353A5054526577 @default.
• W2205513353 hasAuthorship W2205513353A5056487633 @default.
• W2205513353 hasAuthorship W2205513353A5084134381 @default.
• W2205513353 hasAuthorship W2205513353A5087899506 @default.
• W2205513353 hasAuthorship W2205513353A5089871946 @default.
• W2205513353 hasConcept C101762097 @default.
• W2205513353 hasConcept C104317684 @default.
• W2205513353 hasConcept C150194340 @default.
• W2205513353 hasConcept C153911025 @default.
• W2205513353 hasConcept C161733203 @default.

• next