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- W2205988107 abstract "A simple procedure involving simultaneous adsorption and fixation was developed to immobilize β-galactosidase (β-Gal) from Aspergillus oryzae on a polysulfone hollow-fiber membrane module for converting lactose to galacto-oligosaccharides. Polyethylenimine was used as a polyelectrolyte intermediate layer to provide a positively charged character for β-Gal adsorption. β-Gal adsorbed on polyethylenimine layer was then fixed with cross-linking by glutaraldehyde. The concentrations of polyethylenimine, glutaraldehyde, and β-Gal were significant parameters to achieve high activity yield. The optimum concentration of polyethylenimine and glutaraldehyde were determined as 5% (w/v) and 15% (v/v), respectively. The activity yield on the membrane surface increases as the concentration of β-Gal used during adsorption and fixation increased. The highest activity yield obtained was 1.26 IU cm–2 at 5 mg mL–1 of β-Gal concentration. The activity yield is proportionally dependent on the concentration of the β-Gal employed. Moreover, the immobilized β-Gal exhibited higher specific productivity and stable transgalactosylation activity compared with free β-Gal during the lactose-catalyzed conversion reaction." @default.
- W2205988107 created "2016-06-24" @default.
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- W2205988107 date "2015-12-28" @default.
- W2205988107 modified "2023-10-18" @default.
- W2205988107 title "Simultaneous Adsorption and Fixation of <i>Aspergillus oryzae</i> β-Galactosidase on Polyelectrolyte-Layered Polysulfone Hollow-Fiber Membrane" @default.
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- W2205988107 doi "https://doi.org/10.1021/acs.iecr.5b02541" @default.
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