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- W2207444860 abstract "A method is described for the rapid purification of both S-100 protein and calmodulin from crude bovine brain extracts by the use of a fluphenazine-Sepharose affinity column eluted stepwise with decreasing concentrations of free Ca2+. Protein containing only alpha-subunit was purified from preparations of S-100 protein by anion-exchange chromatography. This protein co-migrated with the alpha-subunit of S-100 protein on sodium dodecyl sulphate/urea/polyacrylamide-gel electrophoresis and had an amino acid composition identical with that previously reported for this subunit. The results of u.v.-absorption and fluorescence-emission spectroscopy indicate that the tryptophan residue of the purified alpha-subunit of S-100 protein undergoes a Ca2+-induced change in environment. Measurements of changes in tryptophan fluorescence with increasing Ca2+ concentrations suggest an apparent dissociation constant of the alpha-subunit for Ca2+ of 7 X 10(-5)M in the absence of K+. In the presence of 90mM-K+ this value is increased to 3.4 X 10(-4)M." @default.
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- W2207444860 date "1984-03-15" @default.
- W2207444860 modified "2023-09-27" @default.
- W2207444860 title "Studies on the α-subunit of bovine brain S-100 protein" @default.
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- W2207444860 doi "https://doi.org/10.1042/bj2180691" @default.
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