FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2207486736> ?p ?o ?g. }

• W2207486736 endingPage "63" @default.
• W2207486736 startingPage "58" @default.
• W2207486736 abstract "The ability of sperm cells to develop colored formazan by reduction of MTT was used earlier to develop a spectrophotometric assay to determine the viability of sperm cells for several mammalian species. It was the objective of the present study to visualize microscopically the location of the formazan in boar sperm cells. The MTT staining process of boar sperm cells can be divided into a series of morphological events. Incubation of the sperm cells in the presence of MTT resulted after a few min in a diffuse staining of the midpiece of the sperm cells. Upon further incubation the staining of the midpiece became more intense, and gradually the formation of packed formazan granules became more visible. At the same time, a small formazan stained granule appeared medially on the sperm head, which increased in size during further incubation. After incubation for about 1 h the midpiece granules were intensely stained and more clearly distinct as granules, while aggregation of sperm cells occurred. Around 90% of the sperm cells showed these staining events. At the end of the staining the formazan granules have disappeared from both the sperm cells and medium, whereas formazan crystals appeared as thin crystal threads, that became heavily aggregated in the incubation medium. It was concluded that formazan is taken up by lipid droplets in the cytoplasm. Further, the use of the MTT assay to test for sperm viability should be regarded as a qualitative assay, whereas its practical use at artificial insemination (AI) Stations is limited." @default.
• W2207486736 created "2016-06-24" @default.
• W2207486736 creator A5064014555 @default.
• W2207486736 date "2015-01-01" @default.
• W2207486736 modified "2023-09-25" @default.
• W2207486736 title "Microscopic analysis of MTT stained boar sperm cells." @default.
• W2207486736 cites W1242592451 @default.
• W2207486736 cites W1566271574 @default.
• W2207486736 cites W1597240440 @default.
• W2207486736 cites W1629179716 @default.
• W2207486736 cites W1849819493 @default.
• W2207486736 cites W1967170658 @default.
• W2207486736 cites W1974521042 @default.
• W2207486736 cites W1974825648 @default.
• W2207486736 cites W1982130096 @default.
• W2207486736 cites W1993943341 @default.
• W2207486736 cites W1995853598 @default.
• W2207486736 cites W2017083664 @default.
• W2207486736 cites W2038104564 @default.
• W2207486736 cites W2052704422 @default.
• W2207486736 cites W2055840803 @default.
• W2207486736 cites W2068102085 @default.
• W2207486736 cites W2071997694 @default.
• W2207486736 cites W2101929400 @default.
• W2207486736 cites W2112536806 @default.
• W2207486736 cites W2114918609 @default.
• W2207486736 cites W2118802683 @default.
• W2207486736 cites W2132615347 @default.
• W2207486736 cites W2140927361 @default.
• W2207486736 cites W2147871639 @default.
• W2207486736 cites W2154699860 @default.
• W2207486736 cites W2171061330 @default.
• W2207486736 cites W2171813756 @default.
• W2207486736 cites W2323609614 @default.
• W2207486736 cites W2341769195 @default.
• W2207486736 cites W2398506083 @default.
• W2207486736 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4629571" @default.
• W2207486736 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26623368" @default.
• W2207486736 hasPublicationYear "2015" @default.
• W2207486736 type Work @default.
• W2207486736 sameAs 2207486736 @default.
• W2207486736 citedByCount "5" @default.
• W2207486736 countsByYear W22074867362017 @default.
• W2207486736 countsByYear W22074867362018 @default.
• W2207486736 countsByYear W22074867362020 @default.
• W2207486736 countsByYear W22074867362021 @default.
• W2207486736 crossrefType "journal-article" @default.
• W2207486736 hasAuthorship W2207486736A5064014555 @default.
• W2207486736 hasConcept C105702510 @default.
• W2207486736 hasConcept C143701427 @default.
• W2207486736 hasConcept C1491633281 @default.
• W2207486736 hasConcept C16685009 @default.
• W2207486736 hasConcept C173372395 @default.
• W2207486736 hasConcept C25642318 @default.
• W2207486736 hasConcept C2776892249 @default.
• W2207486736 hasConcept C2777359717 @default.
• W2207486736 hasConcept C2778093475 @default.
• W2207486736 hasConcept C2780301668 @default.
• W2207486736 hasConcept C2780783641 @default.
• W2207486736 hasConcept C2781087480 @default.
• W2207486736 hasConcept C53227056 @default.
• W2207486736 hasConcept C54355233 @default.
• W2207486736 hasConcept C55493867 @default.
• W2207486736 hasConcept C71924100 @default.
• W2207486736 hasConcept C74864618 @default.
• W2207486736 hasConcept C86803240 @default.
• W2207486736 hasConceptScore W2207486736C105702510 @default.
• W2207486736 hasConceptScore W2207486736C143701427 @default.
• W2207486736 hasConceptScore W2207486736C1491633281 @default.
• W2207486736 hasConceptScore W2207486736C16685009 @default.
• W2207486736 hasConceptScore W2207486736C173372395 @default.
• W2207486736 hasConceptScore W2207486736C25642318 @default.
• W2207486736 hasConceptScore W2207486736C2776892249 @default.
• W2207486736 hasConceptScore W2207486736C2777359717 @default.
• W2207486736 hasConceptScore W2207486736C2778093475 @default.
• W2207486736 hasConceptScore W2207486736C2780301668 @default.
• W2207486736 hasConceptScore W2207486736C2780783641 @default.
• W2207486736 hasConceptScore W2207486736C2781087480 @default.
• W2207486736 hasConceptScore W2207486736C53227056 @default.
• W2207486736 hasConceptScore W2207486736C54355233 @default.
• W2207486736 hasConceptScore W2207486736C55493867 @default.
• W2207486736 hasConceptScore W2207486736C71924100 @default.
• W2207486736 hasConceptScore W2207486736C74864618 @default.
• W2207486736 hasConceptScore W2207486736C86803240 @default.
• W2207486736 hasIssue "1" @default.
• W2207486736 hasLocation W22074867361 @default.
• W2207486736 hasLocation W22074867362 @default.
• W2207486736 hasOpenAccess W2207486736 @default.
• W2207486736 hasPrimaryLocation W22074867361 @default.
• W2207486736 hasRelatedWork W1971222893 @default.
• W2207486736 hasRelatedWork W2039088361 @default.
• W2207486736 hasRelatedWork W2065298941 @default.
• W2207486736 hasRelatedWork W2078604156 @default.
• W2207486736 hasRelatedWork W2207486736 @default.
• W2207486736 hasRelatedWork W2783879907 @default.
• W2207486736 hasRelatedWork W2898151124 @default.
• W2207486736 hasRelatedWork W3194991482 @default.
• W2207486736 hasRelatedWork W3196320235 @default.

• next