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- W2207788197 abstract "Introduction: Sentinel lymph node (SLN) biopsy is a common diagnostic procedure for breast cancer. However, because of a shortage of pathology specialists in Japan and discordance between intra-operative and final pathological diagnoses of SLN metastasis, new diagnostic modalities are desperately required. We previously reported a novel method of detecting metastasis in SLNs by a semi-dry dot-blot (SDB) method with 93.3% sensitivity, 96.9% specificity and 96.6% accuracy. Here, we evaluated the efficacy of the SDB method using novel anti-cytokeratin (CK) 18+19 antibodies to diagnose lymph node metastases in breast cancer. Materials and methods: We obtained 73 lymph nodes dissected from 43 patients with breast cancer from July 2013 to May 2014 at Nagasaki University Hospital and the Japanese Red Cross Nagasaki Genbaku Hospital, including 55 sentinel lymph nodes and 18 dissected axillary lymph nodes, which were sliced at 2-mm intervals and washed with phosphate-buffered saline. This lavage fluid was used to diagnose lymph node metastasis by the SDB method; whereas the washed lymph nodes were blindly diagnosed by pathologists using with hematoxylin and eosin (HE the cell pellet was lysed with lysis buffer to extract protein, which was then challenged and visualized with anti-cytokeratin 18 and 19 antibodies, each at 1μg/ml and 0.1μg/ml to distinguish between micrometastases or isolated tumor cells (ITC) and macrometastases; and with chromogen on a dot-blot membrane. Diagnoses based on the SDB method were compared with their HE estimating metastatic amount may be possible with different antibody concentrations. We are producing an SDB kit that uses these antibodies. Citation Format: Ryota Otsubo, Masahiro Oikawa, Hiroshi Hirakawa, Hiroshi Yano, Takeshi Nagayasu. Validation of diagnostic procedure for metastatic lymph nodes in breast cancer using a semi-dry dot-blot method and novel anti-cytokeratin 18+19 antibodies [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P2-01-23." @default.
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- W2207788197 date "2015-04-30" @default.
- W2207788197 modified "2023-09-27" @default.
- W2207788197 title "Abstract P2-01-23: Validation of diagnostic procedure for metastatic lymph nodes in breast cancer using a semi-dry dot-blot method and novel anti-cytokeratin 18+19 antibodies" @default.
- W2207788197 doi "https://doi.org/10.1158/1538-7445.sabcs14-p2-01-23" @default.
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