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- W2208173664 abstract "For the freezing of canine semen, slow to fast cooling rates have been used. Today, there are many ways of achieving this by using manual protocols, automatic protocols or ultrafreezers. Straws were divided in two groups: one batch (10 ejaculates) was automatically frozen, while the other batch was frozen manually. Motility (computer assisted sperm analyzer), morphology and acrosome status (Spermac stain) were evaluated for fresh and frozen-thawed semen. The manual freezing protocol provided higher total (23.85%) and progressive motility values (20.41%) compared to the automatic protocol (13.26 % total motility, progressive motility 9.90%). The acrosome status was strongly influenced by the cryopreservation process, but there were n significant differences between the two ptotocols.When using the CaniPro Freeze extender, a slower cooling rate (the manual protocol) gave better results then a fast one (the automatic protocol)." @default.
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- W2208173664 date "2013-01-01" @default.
- W2208173664 modified "2023-10-18" @default.
- W2208173664 title "Comparison between an automatic and a manual protocol for freezing canine semen." @default.
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