FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2208189757> ?p ?o ?g. }

• W2208189757 endingPage "76" @default.
• W2208189757 startingPage "68" @default.
• W2208189757 abstract "In a proof of concept study, metal-coded affinity tags based on click chemistry (MeCAT-Click) were used to analyze the proteome of Escherichia coli (E. coli) in response to heat stress. This allows high labeling efficiency, high detection sensitivity, and multiplex capabilities, which are pivotal for its application to protein quantification. Two approaches are presented for relative quantification of differentially lanthanide-labeled proteins. The first approach uses isotope-labeling, where ESI–MS was utilized to quantify the differentially labeled proteins from different states of E. coli. With this approach, 14 proteins were found with changed abundance, among them five proteins upregulated. In the second approach, differentially labeled samples were separated by two dimensional gel electrophoresis (2-DE) and scanned by laser ablation inductively coupled plasma mass spectrometry (LA–ICP–MS). Comparison of the signal intensities of the different lanthanides was used to quantify different sample states. Based on this information, ESI–MS was used to identify the proteins with different abundance. The sensitivity of LA–ICP–MS allowed us to find one upregulated protein that was nearly invisible by silver staining (“Probable replication endonuclease from retron EC67”). The advantage of this approach is to locate low abundant proteins with differential expression using LA–ICP–MS, which may be overlooked otherwise. This paper demonstrates the successful application of a novel metal labeling strategy to quantify the proteins from complex biological samples. In comparison with former metal labeling strategies, it reduces the steric hindrance and improves the labeling efficiency during the labeling process, which ensure its successful application. This methodology is compatible with both molecular and elemental mass spectrometry. ESI–MS/MS in combination with software-based search allows the identification and relative quantification of labeled proteins. In addition, LA–ICP–MS helps to locate the labeled proteins in 2-DE gels with superior detection capability, thus, target proteins with low abundance can be precisely followed. Its excellent sensitivity allows one to track the proteins of interest that are barely visible by silver staining." @default.
• W2208189757 created "2016-06-24" @default.
• W2208189757 creator A5002789755 @default.
• W2208189757 creator A5006320283 @default.
• W2208189757 creator A5040506295 @default.
• W2208189757 creator A5063817517 @default.
• W2208189757 creator A5068951265 @default.
• W2208189757 creator A5071052369 @default.
• W2208189757 date "2016-03-01" @default.
• W2208189757 modified "2023-09-25" @default.
• W2208189757 title "Application of MeCAT-Click labeling for protein abundance characterization of E. coli after heat shock experiments" @default.
• W2208189757 cites W1941588397 @default.
• W2208189757 cites W1961642635 @default.
• W2208189757 cites W1975200549 @default.
• W2208189757 cites W2008339907 @default.
• W2208189757 cites W2013176740 @default.
• W2208189757 cites W2025398059 @default.
• W2208189757 cites W2027173346 @default.
• W2208189757 cites W2031948535 @default.
• W2208189757 cites W2048424991 @default.
• W2208189757 cites W2048433235 @default.
• W2208189757 cites W2067395459 @default.
• W2208189757 cites W2072393954 @default.
• W2208189757 cites W2079308952 @default.
• W2208189757 cites W2080489532 @default.
• W2208189757 cites W2091991333 @default.
• W2208189757 cites W2100759349 @default.
• W2208189757 cites W2102750797 @default.
• W2208189757 cites W2105423029 @default.
• W2208189757 cites W2109762248 @default.
• W2208189757 cites W2117725451 @default.
• W2208189757 cites W2128737924 @default.
• W2208189757 cites W2132851761 @default.
• W2208189757 cites W2148192047 @default.
• W2208189757 cites W2158941341 @default.
• W2208189757 cites W2166361214 @default.
• W2208189757 cites W2170707541 @default.
• W2208189757 cites W2313327494 @default.
• W2208189757 cites W3190142423 @default.
• W2208189757 doi "https://doi.org/10.1016/j.jprot.2015.12.010" @default.
• W2208189757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26710723" @default.
• W2208189757 hasPublicationYear "2016" @default.
• W2208189757 type Work @default.
• W2208189757 sameAs 2208189757 @default.
• W2208189757 citedByCount "8" @default.
• W2208189757 countsByYear W22081897572016 @default.
• W2208189757 countsByYear W22081897572017 @default.
• W2208189757 countsByYear W22081897572018 @default.
• W2208189757 countsByYear W22081897572019 @default.
• W2208189757 crossrefType "journal-article" @default.
• W2208189757 hasAuthorship W2208189757A5002789755 @default.
• W2208189757 hasAuthorship W2208189757A5006320283 @default.
• W2208189757 hasAuthorship W2208189757A5040506295 @default.
• W2208189757 hasAuthorship W2208189757A5063817517 @default.
• W2208189757 hasAuthorship W2208189757A5068951265 @default.
• W2208189757 hasAuthorship W2208189757A5071052369 @default.
• W2208189757 hasConcept C104317684 @default.
• W2208189757 hasConcept C104397665 @default.
• W2208189757 hasConcept C12554922 @default.
• W2208189757 hasConcept C162356407 @default.
• W2208189757 hasConcept C165697059 @default.
• W2208189757 hasConcept C185592680 @default.
• W2208189757 hasConcept C21951064 @default.
• W2208189757 hasConcept C2781188995 @default.
• W2208189757 hasConcept C43617362 @default.
• W2208189757 hasConcept C547475151 @default.
• W2208189757 hasConcept C55493867 @default.
• W2208189757 hasConcept C60644358 @default.
• W2208189757 hasConcept C69610077 @default.
• W2208189757 hasConcept C86803240 @default.
• W2208189757 hasConceptScore W2208189757C104317684 @default.
• W2208189757 hasConceptScore W2208189757C104397665 @default.
• W2208189757 hasConceptScore W2208189757C12554922 @default.
• W2208189757 hasConceptScore W2208189757C162356407 @default.
• W2208189757 hasConceptScore W2208189757C165697059 @default.
• W2208189757 hasConceptScore W2208189757C185592680 @default.
• W2208189757 hasConceptScore W2208189757C21951064 @default.
• W2208189757 hasConceptScore W2208189757C2781188995 @default.
• W2208189757 hasConceptScore W2208189757C43617362 @default.
• W2208189757 hasConceptScore W2208189757C547475151 @default.
• W2208189757 hasConceptScore W2208189757C55493867 @default.
• W2208189757 hasConceptScore W2208189757C60644358 @default.
• W2208189757 hasConceptScore W2208189757C69610077 @default.
• W2208189757 hasConceptScore W2208189757C86803240 @default.
• W2208189757 hasFunder F4320322725 @default.
• W2208189757 hasLocation W22081897571 @default.
• W2208189757 hasLocation W22081897572 @default.
• W2208189757 hasOpenAccess W2208189757 @default.
• W2208189757 hasPrimaryLocation W22081897571 @default.
• W2208189757 hasRelatedWork W1983159191 @default.
• W2208189757 hasRelatedWork W1999606269 @default.
• W2208189757 hasRelatedWork W2043901743 @default.
• W2208189757 hasRelatedWork W2788229932 @default.
• W2208189757 hasRelatedWork W2913510406 @default.
• W2208189757 hasRelatedWork W2937474596 @default.
• W2208189757 hasRelatedWork W2952967229 @default.
• W2208189757 hasRelatedWork W3087371238 @default.
• W2208189757 hasRelatedWork W4249269756 @default.

• next