FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2208444107> ?p ?o ?g. }

• W2208444107 endingPage "540" @default.
• W2208444107 startingPage "525" @default.
• W2208444107 abstract "Abstract Single-strand annealing (SSA) is an important homologous recombination mechanism that repairs DNA double strand breaks (DSBs) occurring between closely spaced repeat sequences. During SSA, the DSB is acted upon by exonucleases to reveal complementary sequences that anneal and are then repaired through tail clipping, DNA synthesis, and ligation steps. In baker’s yeast, the Msh DNA mismatch recognition complex and the Sgs1 helicase act to suppress SSA between divergent sequences by binding to mismatches present in heteroduplex DNA intermediates and triggering a DNA unwinding mechanism known as heteroduplex rejection. Using baker’s yeast as a model, we have identified new factors and regulatory steps in heteroduplex rejection during SSA. First we showed that Top3-Rmi1, a topoisomerase complex that interacts with Sgs1, is required for heteroduplex rejection. Second, we found that the replication processivity clamp proliferating cell nuclear antigen (PCNA) is dispensable for heteroduplex rejection, but is important for repairing mismatches formed during SSA. Third, we showed that modest overexpression of Msh6 results in a significant increase in heteroduplex rejection; this increase is due to a compromise in Msh2-Msh3 function required for the clipping of 3′ tails. Thus 3′ tail clipping during SSA is a critical regulatory step in the repair vs. rejection decision; rejection is favored before the 3′ tails are clipped. Unexpectedly, Msh6 overexpression, through interactions with PCNA, disrupted heteroduplex rejection between divergent sequences in another recombination substrate. These observations illustrate the delicate balance that exists between repair and replication factors to optimize genome stability." @default.
• W2208444107 created "2016-06-24" @default.
• W2208444107 creator A5003084107 @default.
• W2208444107 creator A5006280374 @default.
• W2208444107 creator A5038195594 @default.
• W2208444107 creator A5043455538 @default.
• W2208444107 date "2015-12-14" @default.
• W2208444107 modified "2023-10-17" @default.
• W2208444107 title "A Delicate Balance Between Repair and Replication Factors Regulates Recombination Between Divergent DNA Sequences in Saccharomyces cerevisiae" @default.
• W2208444107 cites W1491342176 @default.
• W2208444107 cites W1510511218 @default.
• W2208444107 cites W1565890429 @default.
• W2208444107 cites W1784480918 @default.
• W2208444107 cites W1851841933 @default.
• W2208444107 cites W1905978644 @default.
• W2208444107 cites W1966616557 @default.
• W2208444107 cites W1966976551 @default.
• W2208444107 cites W1967205646 @default.
• W2208444107 cites W1967667819 @default.
• W2208444107 cites W1968206926 @default.
• W2208444107 cites W1980462742 @default.
• W2208444107 cites W1984131593 @default.
• W2208444107 cites W1984354326 @default.
• W2208444107 cites W1984693763 @default.
• W2208444107 cites W1988134858 @default.
• W2208444107 cites W1990828107 @default.
• W2208444107 cites W1993026474 @default.
• W2208444107 cites W1993073412 @default.
• W2208444107 cites W1993925622 @default.
• W2208444107 cites W1995649118 @default.
• W2208444107 cites W2000422680 @default.
• W2208444107 cites W2004297150 @default.
• W2208444107 cites W2009119431 @default.
• W2208444107 cites W2009319344 @default.
• W2208444107 cites W2012816024 @default.
• W2208444107 cites W2013303121 @default.
• W2208444107 cites W2015532523 @default.
• W2208444107 cites W2016544300 @default.
• W2208444107 cites W2019551788 @default.
• W2208444107 cites W2023563387 @default.
• W2208444107 cites W2024172421 @default.
• W2208444107 cites W2026388436 @default.
• W2208444107 cites W2028785832 @default.
• W2208444107 cites W2029003189 @default.
• W2208444107 cites W2031142206 @default.
• W2208444107 cites W2032587846 @default.
• W2208444107 cites W2039950254 @default.
• W2208444107 cites W2044569209 @default.
• W2208444107 cites W2045853725 @default.
• W2208444107 cites W2046144388 @default.
• W2208444107 cites W2052401924 @default.
• W2208444107 cites W2052547134 @default.
• W2208444107 cites W2053223320 @default.
• W2208444107 cites W2055550132 @default.
• W2208444107 cites W2055948417 @default.
• W2208444107 cites W2058388509 @default.
• W2208444107 cites W2060354616 @default.
• W2208444107 cites W2066437830 @default.
• W2208444107 cites W2071761362 @default.
• W2208444107 cites W2075549762 @default.
• W2208444107 cites W2076629799 @default.
• W2208444107 cites W2076744932 @default.
• W2208444107 cites W2078502658 @default.
• W2208444107 cites W2082557856 @default.
• W2208444107 cites W2083751217 @default.
• W2208444107 cites W2085648753 @default.
• W2208444107 cites W2091512138 @default.
• W2208444107 cites W2091578308 @default.
• W2208444107 cites W2093452293 @default.
• W2208444107 cites W2094020226 @default.
• W2208444107 cites W2094981589 @default.
• W2208444107 cites W2099974826 @default.
• W2208444107 cites W2101665191 @default.
• W2208444107 cites W2102259410 @default.
• W2208444107 cites W2102561949 @default.
• W2208444107 cites W2104161593 @default.
• W2208444107 cites W2109714973 @default.
• W2208444107 cites W2112819037 @default.
• W2208444107 cites W2113548088 @default.
• W2208444107 cites W2114700051 @default.
• W2208444107 cites W2114968751 @default.
• W2208444107 cites W2122259228 @default.
• W2208444107 cites W2124295966 @default.
• W2208444107 cites W2127156205 @default.
• W2208444107 cites W2127566231 @default.
• W2208444107 cites W2128635872 @default.
• W2208444107 cites W2128948249 @default.
• W2208444107 cites W2129225238 @default.
• W2208444107 cites W2132706510 @default.
• W2208444107 cites W2137334007 @default.
• W2208444107 cites W2138539875 @default.
• W2208444107 cites W2144393305 @default.
• W2208444107 cites W2146503498 @default.
• W2208444107 cites W2148034980 @default.
• W2208444107 cites W2151162505 @default.
• W2208444107 cites W2158151897 @default.
• W2208444107 cites W2158602985 @default.
• W2208444107 cites W2158658867 @default.

• next