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- W2208596989 abstract "Abstract A water-soluble polysaccharide, LBLP5-A, was isolated from Lycium barbarum leaves, which is widely used as a medicine in Asia. The average molecular weight of LBLP5-A is 11.33 × 10 4 Da. With O-type carbohydrate–peptide linkage in its structure, LBLP5-A afforded glycan fractions of LBLP5-A-OL1, LBLP5-A-OL2, LBLP5-A-OL3, and LBLP5-A-OL4 acquired by β-elimination reaction. Detailed structure patterns of LBLP5-A-OL1 were characterized by monosaccharide composition analysis, methylation analysis, partial acid hydrolysis, Smith degradation, one- or two-dimensional (1D/2D) nuclear magnetic resonance (NMR), and electrospray ionization–mass spectrometry (ESI–MS). LBLP5-A-OL1 was identified to be a highly branched polysaccharide with a backbone of (1 → 3)-linked Gal, which was partially substituted at its O-6 position. These branches, with arabinose and galactose terminals, were assigned to be (1 → 3)-linked Gal, (1 → 4)-linked Gal, (1 → 3)-linked Ara f , (1 → 5)-linked Ara f , and (1 → 2, 4)-linked Rha f. LBLP5-A-OL3 and LBLP5-A-OL4 were determined to be a series of oligosaccharides that share the general structure pattern of Ara n1 → Gal n2 → Rha n3 ( n 1 = 0–6, n 2 = 0–8, n 3 = 0–1) by ESI–MS. Moreover, LBLP5-A exhibited excellent antioxidativity, concerning the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radicals in vitro ." @default.
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- W2208596989 date "2016-02-01" @default.
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- W2208596989 title "Isolation, structural characterization, and antioxidativity of polysaccharide LBLP5-A from Lycium barbarum leaves" @default.
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- W2208596989 doi "https://doi.org/10.1016/j.procbio.2015.11.013" @default.
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