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- W2209936224 abstract "Abstract A quantitative method is described for the determination of gamma globulin in the cerebrospinal fluid. The technique is based on chromatographic separation of the gamma globulin from other proteins using DEAE-Sephadex anion exchanger. Through the molecular sieving action of the Sephadex, the low molecular weight compounds which interfere in the subsequent step of the determination are retarded on the column. Measurement of the eluted gamma globulin is then carried out in a Zeiss spectrophotometer at 220 mμ. Under conditions of the method, 0.5 ml. cerebrospinal fluid, and 0.025M phosphate buffer of pH 7.0, the gamma globulin is eluted from the gel, whereas other proteins and compounds of the fluid which interfere in the subsequent step of the procedure remain adsorbed on DEAE-Sephadex gel. The separated gamma globulin is assayed spectrophotometrically at 220 mμ and is read directly in milligrams per cent from a standard curve. For preparation of the standard curve, the commercial gamma globulin was purified. After chromatographic treatment, the gamma globulin is free of all other proteins as demonstrated by electrophoresis. The experiments carried out were with gamma globulin, with the separation from other proteins with the use of the serum preparation Versatol, duplicate determinations of cerebrospinal fluid, and recovery of gamma globulin added to cerebrospinal fluid. All the experiments indicate that the method is accurate. It is also simple and fast, and therefore eminently suitable for clinical laboratory use. It is recommended that the gamma globulin values should be related to total protein, since the percentage of gamma of the total protein better reflects the changes which occur in multiple sclerosis than the gamma globulin values alone." @default.
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- W2209936224 title "Determination of gamma globulin in the cerebrospinal fluid by quantitative chromatography." @default.
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