FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2210080482> ?p ?o ?g. }

• W2210080482 endingPage "11" @default.
• W2210080482 startingPage "1" @default.
• W2210080482 abstract "Dehydroepiandrosterone (DHEA) is a precursor of androgen synthesis whose action is partially exerted through its metabolites. 7-Oxo-dehydroepiandrosterone (7-oxo-DHEA) is a common DHEA metabolite, non-convertible to androgens, which constitutes a promising therapeutic strategy for multiple conditions. Sertoli cells (SCs) are responsible for the support of spermatogenesis, having unique metabolic characteristics strongly modulated by androgens. Consequently, disruptions in androgen synthesis compromise SCs function and hence male fertility. We aimed to evaluate the effects of DHEA and 7-oxo-DHEA in human SCs (hSCs) metabolism and oxidative profile. To do so, hSCs were exposed to increasing concentrations of DHEA and 7-oxo-DHEA (0.025, 1 and 50 μM) that revealed to be non-cytotoxic in these experimental conditions. We measured hSCs metabolites consumption/production by (1)H NMR, the protein expression levels of key players of the glycolytic pathway by Western blot as well as the levels of carbonyl groups, nitration and lipid peroxidation by Slot blot. The obtained data demonstrated that 7-oxo-DHEA is a more potent metabolic modulator than DHEA since it increased hSCs glycolytic flux. DHEA seem to redirect hSCs metabolism to the Krebs cycle, while 7-oxo-DHEA has some inhibitory effect in this path. The highest 7-oxo-DHEA concentrations (1 and 50 μM) also increased lactate production, which is of extreme relevance for the successful progression of spermatogenesis in vivo. None of these steroids altered the intracellular oxidative profile of hSCs, illustrating that, at the concentrations used they do not have pro- nor antioxidant actions in hSCs. Our study represents a further step in the establishment of safe doses of DHEA and 7-oxo-DHEA to hSCs, supporting its possible use in hormonal and non-hormonal therapies against male reproductive problems." @default.
• W2210080482 created "2016-06-24" @default.
• W2210080482 creator A5019042215 @default.
• W2210080482 creator A5021022567 @default.
• W2210080482 creator A5022114121 @default.
• W2210080482 creator A5062327376 @default.
• W2210080482 creator A5062804840 @default.
• W2210080482 creator A5064605317 @default.
• W2210080482 creator A5071085717 @default.
• W2210080482 creator A5073431252 @default.
• W2210080482 creator A5091691731 @default.
• W2210080482 date "2015-11-01" @default.
• W2210080482 modified "2023-10-17" @default.
• W2210080482 title "Dehydroepiandrosterone and 7-oxo-dehydroepiandrosterone in male reproductive health: Implications of differential regulation of human Sertoli cells metabolic profile" @default.
• W2210080482 cites W1511003303 @default.
• W2210080482 cites W1567614586 @default.
• W2210080482 cites W1585400552 @default.
• W2210080482 cites W1616394907 @default.
• W2210080482 cites W1924799945 @default.
• W2210080482 cites W1944318342 @default.
• W2210080482 cites W1967655383 @default.
• W2210080482 cites W1968751935 @default.
• W2210080482 cites W1969078349 @default.
• W2210080482 cites W1969438694 @default.
• W2210080482 cites W1978305871 @default.
• W2210080482 cites W1985168335 @default.
• W2210080482 cites W1991069718 @default.
• W2210080482 cites W1991149714 @default.
• W2210080482 cites W1993217396 @default.
• W2210080482 cites W1996202353 @default.
• W2210080482 cites W1998106197 @default.
• W2210080482 cites W2005534385 @default.
• W2210080482 cites W2013074188 @default.
• W2210080482 cites W2013572506 @default.
• W2210080482 cites W2014577850 @default.
• W2210080482 cites W2015863848 @default.
• W2210080482 cites W2021082765 @default.
• W2210080482 cites W2021824743 @default.
• W2210080482 cites W2022894463 @default.
• W2210080482 cites W2031754021 @default.
• W2210080482 cites W2031981194 @default.
• W2210080482 cites W2034581879 @default.
• W2210080482 cites W2035779548 @default.
• W2210080482 cites W2042151892 @default.
• W2210080482 cites W2048609616 @default.
• W2210080482 cites W2055116762 @default.
• W2210080482 cites W2056317493 @default.
• W2210080482 cites W2056865735 @default.
• W2210080482 cites W2058350561 @default.
• W2210080482 cites W2059183204 @default.
• W2210080482 cites W2059643569 @default.
• W2210080482 cites W2059856147 @default.
• W2210080482 cites W2064418467 @default.
• W2210080482 cites W2070617807 @default.
• W2210080482 cites W2075216359 @default.
• W2210080482 cites W2076466972 @default.
• W2210080482 cites W2076477755 @default.
• W2210080482 cites W2076615745 @default.
• W2210080482 cites W2081019639 @default.
• W2210080482 cites W2081491337 @default.
• W2210080482 cites W2082395903 @default.
• W2210080482 cites W2084377042 @default.
• W2210080482 cites W2088926355 @default.
• W2210080482 cites W2104477642 @default.
• W2210080482 cites W2105069022 @default.
• W2210080482 cites W2111674612 @default.
• W2210080482 cites W2112066669 @default.
• W2210080482 cites W2119132931 @default.
• W2210080482 cites W2120158027 @default.
• W2210080482 cites W2124813430 @default.
• W2210080482 cites W2130267710 @default.
• W2210080482 cites W2131325881 @default.
• W2210080482 cites W2132809876 @default.
• W2210080482 cites W2142451890 @default.
• W2210080482 cites W2145074286 @default.
• W2210080482 cites W2147746176 @default.
• W2210080482 cites W2148746144 @default.
• W2210080482 cites W2155666326 @default.
• W2210080482 cites W2155801632 @default.
• W2210080482 cites W2161128788 @default.
• W2210080482 cites W2164229548 @default.
• W2210080482 cites W2169423794 @default.
• W2210080482 cites W2327091725 @default.
• W2210080482 cites W27403733 @default.
• W2210080482 cites W3023236327 @default.
• W2210080482 cites W4210540581 @default.
• W2210080482 cites W4246234200 @default.
• W2210080482 cites W4385616783 @default.
• W2210080482 doi "https://doi.org/10.1016/j.jsbmb.2015.06.004" @default.
• W2210080482 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26134425" @default.
• W2210080482 hasPublicationYear "2015" @default.
• W2210080482 type Work @default.
• W2210080482 sameAs 2210080482 @default.
• W2210080482 citedByCount "11" @default.
• W2210080482 countsByYear W22100804822016 @default.
• W2210080482 countsByYear W22100804822017 @default.
• W2210080482 countsByYear W22100804822018 @default.
• W2210080482 countsByYear W22100804822020 @default.

• next