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- W2210400282 abstract "Bone formation is a complex process that requires a wide array of molecules in order to properly occur. Mesenchymal stem cells, which can be differentiated into adipocytes, myoblasts, fibroblasts and chondrocytes, can also give raise to osteoblasts under the activation of osteoblastic factors. Previous in vitro studies have shown that the transcription factor family NFI, regulates the mRNA expression of IGFBP5, stimulator of osteoblast proliferation and differentiation, in human osteoblasts. Moreover, the knockout Nfix model displays impaired endochondral ossification. We evaluated the role of the Nfi gene family in osteoblast differentiation using the MC3T3-E1 preosteoblast in vitro model for osteoblast differentiation. Nfix mRNA levels increased during MC3T3-E1 osteoblast differentiation induced by ascorbic acid and β-glycerophosphate. Stable knockdown of Nfix in MC3T3-EI cells was accomplished using lentiviral shRNA vectors. Cells stably transduced with a validated shRNA vector targeting Nfix or a nontargeting shRNA vector grown in differentiation media were observed to have delayed mineralization compared to control cells. mRNA transcript levels of osteocalcin (Ocn), bone sialoprotein (Bsp) and Runt-related transcription factor 2 (Runx-2) were significantly decreased in Nfix deficient calvarial osteoblasts. Promoter-luciferase reporter assays also showed that transient transfection of MC3T3-E1 cells with a plasmid" @default.
- W2210400282 created "2016-06-24" @default.
- W2210400282 creator A5083329651 @default.
- W2210400282 date "2013-01-01" @default.
- W2210400282 modified "2023-09-27" @default.
- W2210400282 title "Regulation of IGFBP-5 and Osteoblast Functions by Nuclear Factor I" @default.
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- W2210400282 hasPublicationYear "2013" @default.
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