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- W2210738511 abstract "Abstract Unculturable bacterial communities provide a rich source of biocatalysts, but their experimental discovery by functional metagenomics is difficult, because the odds are stacked against the experimentor. Here we demonstrate functional screening of a million-membered metagenomic library in microfluidic picolitre droplet compartments. Using bait substrates, new hydrolases for sulfate monoesters and phosphotriesters were identified, mostly based on promiscuous activities presumed not to be under selection pressure. Spanning three protein superfamilies, these break new ground in sequence space: promiscuity now connects enzymes with only distantly related sequences. Most hits could not have been predicted by sequence analysis, because the desired activities have never been ascribed to similar sequences, showing how this approach complements bioinformatic harvesting of metagenomic sequencing data. Functional screening of a library of unprecedented size with excellent assay sensitivity has been instrumental in identifying rare genes constituting catalytically versatile hubs in sequence space as potential starting points for the acquisition of new functions." @default.
- W2210738511 created "2016-06-24" @default.
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- W2210738511 date "2015-12-07" @default.
- W2210738511 modified "2023-10-03" @default.
- W2210738511 title "Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics" @default.
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- W2210738511 cites W1873385545 @default.
- W2210738511 cites W1945992259 @default.
- W2210738511 cites W1967496291 @default.
- W2210738511 cites W1969733451 @default.
- W2210738511 cites W1970051672 @default.
- W2210738511 cites W1975373045 @default.
- W2210738511 cites W1982554906 @default.
- W2210738511 cites W1984180361 @default.
- W2210738511 cites W1987370810 @default.
- W2210738511 cites W1989179901 @default.
- W2210738511 cites W1993554361 @default.
- W2210738511 cites W1995816579 @default.
- W2210738511 cites W1996471792 @default.
- W2210738511 cites W1998238795 @default.
- W2210738511 cites W1998538921 @default.
- W2210738511 cites W1999106047 @default.
- W2210738511 cites W2001641653 @default.
- W2210738511 cites W2004258912 @default.
- W2210738511 cites W2014916384 @default.
- W2210738511 cites W2017364432 @default.
- W2210738511 cites W2021278578 @default.
- W2210738511 cites W2029886517 @default.
- W2210738511 cites W2030925785 @default.
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- W2210738511 cites W2034452335 @default.
- W2210738511 cites W2038840577 @default.
- W2210738511 cites W2044172420 @default.
- W2210738511 cites W2045366126 @default.
- W2210738511 cites W2047067740 @default.
- W2210738511 cites W2054457861 @default.
- W2210738511 cites W2059018615 @default.
- W2210738511 cites W2060797027 @default.
- W2210738511 cites W2061657565 @default.
- W2210738511 cites W2062461675 @default.
- W2210738511 cites W2064754363 @default.
- W2210738511 cites W2066841065 @default.
- W2210738511 cites W2070040197 @default.
- W2210738511 cites W2078782272 @default.
- W2210738511 cites W2087230704 @default.
- W2210738511 cites W2091130149 @default.
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- W2210738511 cites W2093200394 @default.
- W2210738511 cites W2095071403 @default.
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- W2210738511 doi "https://doi.org/10.1038/ncomms10008" @default.
- W2210738511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4686663" @default.
- W2210738511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26639611" @default.
- W2210738511 hasPublicationYear "2015" @default.
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