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- W221110194 abstract "△Gp5,M and N genes of porcine reproductive and respiratory syndrome virus(PRRSV) of American strain VR2332 were amplified by RT-PCR,and the tandem △Gp5/M/N gene was amplified by recombinant PCR.The △Gp5/M/N gene was cloned into the pET-30a(+) expression vector.After identification by PCR and digestion with restriction endonuclease,the recombinant plasmid was transformed into BL21(DE3) pLysS.The expression of △Gp5/M/N gene was confirmed by analyses using SDS-PAGE and Western-blotting,indicating that recombinant △Gp5/M/N protein existed in the form of inclusion bodies.The purified protein could react specifically with PRRSV positive sera.The results showed that recombinant △Gp5/M/N protein expressed in E.coli had good reactogenicity." @default.
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- W221110194 date "2009-01-01" @default.
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- W221110194 title "Cloning and prokaryotic expression of tandem ΔGp5/M/N gene of porcine reproductive and respiratory syndrome virus." @default.
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