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- W2212073025 abstract "In situ hybridization technology is a tool to localize nucleic acid molecules in plant and animal cells. Several reviews and comparison studies have outlined the general methods. The two main approaches involve either radioactive or nonradioactive-labeled probes. The choice of which procedure to use is usually a compromise decision weighing the good and bad characteristics of each. The use of radioactive probes is increasingly more bothersome because of the monitoring equipment, special procedures, and the expense of handling, storage, and waste disposal. Radioactive procedures will probably never be abandoned completely, but when starting an in situ study for the first time, nonradioactive methods should probably be considered carefully as a first approach. The most widely used nonradioactive methods use probes labeled with biotin or digoxigenin. Both methods are safe and stable, but neither is considered to have the high sensitivity of radioactive probes." @default.
- W2212073025 created "2016-06-24" @default.
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- W2212073025 date "1993-01-01" @default.
- W2212073025 modified "2023-09-27" @default.
- W2212073025 title "Non-radioactive In Situ RNA Hybridisation Using Digoxigenin and an Application for Co-localisation Studies with Radioisotopes" @default.
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- W2212073025 doi "https://doi.org/10.1016/b978-0-12-461020-0.50013-5" @default.
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