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- W2212779851 abstract "Publisher Summary This chapter discusses methods that have developed for the localization of cyclic nucleotide phosphodiesterase activity: the enzyme cytochemical method of Shanta et al. and the correlating biochemical method of Roof et al. using quick freezing and deep etching. The procedure of Shanta et al. 1 is based on light microscopy. In 1982, Roof et al. tried to demonstrate the localization of the cGMPdependent, light-activated phosphodiesterase with the quick-frozen and deep-etched technique on the disk membranes. Although there are no theoretical disadvantages in this procedure, it failed to demonstrate the differences among the different groups despite much elaborate work. Each method for localizing cyclic nucleotide phosphodiesterase has its advantages and disadvantages. It is advisable to select the best method for the purpose of the study or to use a combination of methods to reveal the localization." @default.
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- W2212779851 date "1988-01-01" @default.
- W2212779851 modified "2023-09-27" @default.
- W2212779851 title "[45] Cyclic nucleotide phosphodiesterase activity: Histochemical and cytochemical methods" @default.
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- W2212779851 doi "https://doi.org/10.1016/0076-6879(88)59047-x" @default.
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