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• W2212968523 abstract "HomePlant DiseaseVol. 100, No. 6First Report of Botryotinia fuckeliana Causing Gray Mold of Annual Ryegrass (Lolium multiflorum) in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Botryotinia fuckeliana Causing Gray Mold of Annual Ryegrass (Lolium multiflorum) in ChinaL. H. Xue, C. J. Li, Y. Liu, W. X. Wu, L. Zhang, X. Q. Huang, and W. ZhangL. H. XueSearch for more papers by this author, C. J. LiSearch for more papers by this author, Y. LiuSearch for more papers by this author, W. X. WuSearch for more papers by this author, L. ZhangSearch for more papers by this author, X. Q. HuangSearch for more papers by this author, and W. ZhangSearch for more papers by this authorAffiliationsAuthors and Affiliations L. H. Xue , Plant Protection Institute, Sichuan Academy of Agricultural Science, Chengdu 610066, China; and State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China C. J. Li , State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China Y. Liu W. X. Wu L. Zhang X. Q. Huang , Plant Protection Institute, Sichuan Academy of Agricultural Science, Chengdu 610066, China W. Zhang , Lingnan Normal College, Zhanjiang 5240248, China. Published Online:16 Mar 2016https://doi.org/10.1094/PDIS-08-15-0955-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Annual ryegrass (Lolium multiflorum) is a high-yield, high-quality forage grass widely used in animal husbandry and fishery, and cultivated as forage grass varieties in Sichuan Province. In January 2015, a new disease was observed in a commercial field of ryegrass in both Hongya and Pengzhou counties, Sichuan Province, China. Approximately 3 to 5% of plants in these fields were affected. Small, gray spots appeared on the basal portion of leaves first, eventually spreading to cover entire leaves. Under wet, humid conditions, the disease developed rapidly, and gray mycelium and conidia were visible on both leaf surfaces with gray spots. However, under dry and higher temperatures conditions, the disease developed slowly or even became quiescent. For isolation, diseased leaves were surface-disinfested in 5% NaOCl solution for 3 min, 75% ethanol solution for 30s, rinsed thrice in sterilized distilled water, air dried, plated on potato dextrose agar (PDA), and incubated at 25°C for 3 days in the dark. Ten fungal strains were isolated from these symptomatic leaves collected from 2 different fields. For pathogenicity tests, leaves of field-grown annual ryegrasses (‘Spendor’) were inoculated on moist paper towels in an enamelware tray with PDA plugs (4-mm diam.) colonized with the pathogen. Noncolonized PDA plugs served as controls. Three treatments with the same isolate (RHY5) and 10 healthy and consistent leaves per treatment were used. Trays were covered individually with transparent plastic films for 3 days after inoculation to maintain high relative humidity in a greenhouse at 19 to 24°C. The foliar lesions similar to those in the field developed on leaves 2 days after inoculation, and after 3 days, 100% of the leaves were severely infected; control plants remained healthy. The same pathogen was consistently reisolated from the symptomatic tissues. Mycelia were initially hyaline and white, becoming dark gray after 72 h cultured. The conidia produced on PDA were one-celled, ellipsoid or ovoid, 6.1 to 11.3 × 4.8 to 8.5 μm (average 9.8 × 7.3 μm), was similar to the recently reported isolate GM7 (6.1 to 10.6 × 5.1 to 7.8 μm) (Aktaruzzaman et al. 2015). Microsclerotia were round, spherical or irregular in shape, and 1.3 to 3.2 × 1.5 to 3.6 mm. These morphological characteristics were consistent with those of Botrytis cinerea (Zhang 2006), anamorph of Botryotinia fuckeliana. Teleomorph was not observed. Strain RHY5 was selected as a representative for molecular identification. The ITS region of rDNA was amplified with primers ITS1/ITS4 and sequenced. BLAST analysis of the 515-bp segment (GenBank Accessions No. KT582111) showed 100% identity with the sequence of Botryotinia fuckeliana (teleomorph of B. cinerea). To further identify the species of B. fuckeliana, three nuclear protein-coding genes (G3PDH, HSP60, and RPB2) (Staats et al. 2005) were sequenced and the sequences (GenBank Accessions No. KT378438, KT378439, and KT378440, respectively) all showed 100% identity with those of B. fuckeliana. To our knowledge, this is the first report of B. fuckeliana causing gray mold on L. multiflorum in China.References:Aktaruzzaman, M. et al. 2015. Plant Dis. 99:1865. Link, ISI, Google ScholarStaats, M., et al. 2005. Mol. Biol. Evol. 22:333. https://doi.org/10.1093/molbev/msi020 Crossref, ISI, Google ScholarZhang, Z. Y. 2006. Flora Fungorum Sinicorum. 26. Botrytis, Ramularia. Science Press, Beijing. Google ScholarThis research was financially supported by the Special Fund for Agro-Scientific Research in the Public Interest (201303057).DetailsFiguresLiterature CitedRelated Vol. 100, No. 6 June 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 12 May 2016Published: 16 Mar 2016First Look: 19 Dec 2015Accepted: 13 Dec 2015 Pages: 1236-1236 Information© 2016 The American Phytopathological SocietyCited byBotryotinia fuckeliana (grey mould-rot)CABI Compendium, Vol. CABI CompendiumFirst Report of Botrytis cinerea Causing Soft Rots in Trifolium repens in ChinaL. H. Xue, Y. Liu, and C. J. Li6 April 2017 | Plant Disease, Vol. 101, No. 6" @default.
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• W2212968523 title "First Report of <i>Botryotinia fuckeliana</i> Causing Gray Mold of Annual Ryegrass (<i>Lolium multiflorum</i>) in China" @default.
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