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- W2213197281 abstract "Escherichia coli 5′-nucleotidase is a two-domain enzyme exhibiting a unique 96° domain motion that is required for catalysis. Here we present an integrated structural biology study that combines DEER distance distributions with structural information from X-ray crystallography and computational biology to describe the population of presumably almost isoenergetic open and closed states in solution. Ensembles of models that best represent the experimental distance distributions are determined by a Monte Carlo search algorithm. As a result, predominantly open conformations are observed in the unliganded state indicating that the majority of enzyme molecules await substrate binding for the catalytic cycle. The addition of a substrate analog yields ensembles with an almost equal mixture of open and closed states. Thus, in the presence of substrate, efficient catalysis is provided by the simultaneous appearance of open conformers (binding substrate or releasing product) and closed conformers (enabling the turnover of the substrate)." @default.
- W2213197281 created "2016-06-24" @default.
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- W2213197281 date "2016-01-01" @default.
- W2213197281 modified "2023-10-14" @default.
- W2213197281 title "Characterization of the Domain Orientations of E. coli 5′-Nucleotidase by Fitting an Ensemble of Conformers to DEER Distance Distributions" @default.
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- W2213197281 doi "https://doi.org/10.1016/j.str.2015.11.007" @default.
- W2213197281 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4706771" @default.
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