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- W2213488733 abstract "This chapter describes the estimation of 3-deoxy-2-ketoaldonic acids. In estimation with thiobarbituric acid, treatment of 3-deoxy-2-ketoaldonic acids with periodate yields 2,4-dioxobutyrate, which condenses with 2-thiobarbituric acid to give a red dye having an absorption maximum at 549 nm. Only free— that is, unsubstituted, 3-deoxy-2-ketoaldonic acids can be estimated reliably with periodate/thiobarbiturate. Indeed, to obtain quantitative results, one molar equivalent of 2,4-dioxobutyrate “β-formyl pyruvate” must be released during the oxidation reaction, and this reaction must take place at a rate considerably greater than the destruction of the dioxobutyrate itself by periodate. In the case of phosphorylated aldulosonic acids with 5–8 carbon atoms dephosphorylation is carried out with acid phosphatase prior to the periodate cleavage; the incubation with the enzyme is carried out in a volume of 0.1–0.3 ml, which is then diluted to the appropriate volume with the cold NaIO4/H2SO4 solution. In the case of 5-0-substituted 3-deoxy-2-ketooctonic acids, the molar absorption coefficient was found to be about 13,000 for both compounds, and it is probable that this value will be valid for all 5-0-substituted 3-deoxyoctulosonates. The molar absorption coefficient in the conditions of the cold acid method has not been determined. In estimation with diphenylamine, 3-Deoxy-D-manno-octulosonic acid is heated with diphenylamine in acid solution; a condensation product of unknown composition is formed whose absorption is used for the estimation of the aldulosonate. The method can be applied successfully for the accurate estimation of 5-0-glycosylated 3-deoxyoctulosonic acids, and very probably, to that of free and 0-substituted 3-deoxy-2-ketoaldonic acids in general, provided that the 0-substituent is acid labile." @default.
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- W2213488733 date "1975-01-01" @default.
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- W2213488733 title "[6] Estimation of 3-deoxy-2-ketoaldonic acids" @default.
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- W2213488733 doi "https://doi.org/10.1016/s0076-6879(75)41008-4" @default.
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