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- W2213760023 abstract "Publisher Summary This chapter discusses methods to determine protein-protein relationships in ribosomes using protection from chemical and enzymic modification. Escherichia coli is used throughout in the chapter as an example to illustrate the methods of chemical and enzymic protection for detecting protein-protein relationships. The 30 S bacterial ribosome can be completely reassembled from a mixture of individually purified 30 S proteins and the component 16 S RNA. The technique to construct many different intermediate protein-RNA complexes makes it possible to compare two separate protein-RNA complexes differing in protein content by only a single protein. The protection approach involves the modification of two such protein-RNA complexes, either with a protein selective reagent 5 or a proteolytic enzyme, and analysis of the modification products to determine whether or not the presence of the extra protein in the second complex protected any of the other component proteins from modification. To illustrate the basic procedure for proteolytic protection, the chapter presents a specific series of experiments." @default.
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- W2213760023 date "1979-01-01" @default.
- W2213760023 modified "2023-10-17" @default.
- W2213760023 title "[46] Some methods using protection from chemical and enzymic modification to determine protein-protein relationships in ribosomes" @default.
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- W2213760023 doi "https://doi.org/10.1016/0076-6879(79)59115-0" @default.
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