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• W2213825066 endingPage "652" @default.
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• W2213825066 abstract "Equinatoxin II (EqtII), a eukaryotic pore-forming toxin, lyses cell membranes through a mechanism involving the insertion of its N-terminal α-helix into the membrane. EqtII pore formation is dependent on sphingomyelin (SM), although cholesterol (Chol) and membrane microdomains have also been suggested to enhance its activity. We have investigated the mechanism of EqtII binding and insertion by using neutron reflection to determine the structures of EqtII–membrane assemblies in situ. EqtII has several different modes of binding to membranes depending on the lipid composition. In pure dimyristoyl-phosphatidylcholine (DMPC) membranes, EqtII interacts weakly and reversibly with the lipid head groups in an orientation approximately parallel to the membrane surface. The presence of sphingomyelin (SM) gives rise to a more upright orientation of EqtII, but Chol is required for insertion into the core of the membrane. Cooling the EqtII–lipid assembly below the lipid phase transition temperature leads to deep water penetration and a significant reduction in the extension of the protein outside the membrane, indicating that phase-separation plays a role in EqtII pore-formation. An inactive double-cysteine mutant of EqtII in which the α-helix is covalently tethered to the rest of the protein, interacts only reversibly with all the membranes. Releasing the α-helix in situ by reduction of the disulphide bridge, however, causes the mutant protein to penetrate in DMPC–SM–Chol membranes in a manner identical to that of the wild-type protein. Our results help clarify the early steps in pore formation by EqtII and highlight the valuable information on protein–membrane interactions available from neutron reflection measurements." @default.
• W2213825066 created "2016-06-24" @default.
• W2213825066 creator A5016501395 @default.
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• W2213825066 date "2016-04-01" @default.
• W2213825066 modified "2023-09-26" @default.
• W2213825066 title "Neutron reflection study of the interaction of the eukaryotic pore-forming actinoporin equinatoxin II with lipid membranes reveals intermediate states in pore formation" @default.
• W2213825066 cites W1502827303 @default.
• W2213825066 cites W1963734677 @default.
• W2213825066 cites W1965590736 @default.
• W2213825066 cites W1965642539 @default.
• W2213825066 cites W1967514838 @default.
• W2213825066 cites W1968615540 @default.
• W2213825066 cites W1973046876 @default.
• W2213825066 cites W1977183850 @default.
• W2213825066 cites W1984571919 @default.
• W2213825066 cites W1990960974 @default.
• W2213825066 cites W1991146845 @default.
• W2213825066 cites W1994906221 @default.
• W2213825066 cites W1995562471 @default.
• W2213825066 cites W1998187294 @default.
• W2213825066 cites W1999745298 @default.
• W2213825066 cites W2002459713 @default.
• W2213825066 cites W2002621456 @default.
• W2213825066 cites W2005403912 @default.
• W2213825066 cites W2005444157 @default.
• W2213825066 cites W2009308784 @default.
• W2213825066 cites W2010995681 @default.
• W2213825066 cites W2017698288 @default.
• W2213825066 cites W2018359124 @default.
• W2213825066 cites W2018532807 @default.
• W2213825066 cites W2020593261 @default.
• W2213825066 cites W2021551854 @default.
• W2213825066 cites W2025829221 @default.
• W2213825066 cites W2028149448 @default.
• W2213825066 cites W2031450757 @default.
• W2213825066 cites W2032357789 @default.
• W2213825066 cites W2032877221 @default.
• W2213825066 cites W2034060939 @default.
• W2213825066 cites W2035930530 @default.
• W2213825066 cites W2038497192 @default.
• W2213825066 cites W2047450781 @default.
• W2213825066 cites W2052069688 @default.
• W2213825066 cites W2052090852 @default.
• W2213825066 cites W2052589221 @default.
• W2213825066 cites W2054127621 @default.
• W2213825066 cites W2056235355 @default.
• W2213825066 cites W2059890545 @default.
• W2213825066 cites W2060250212 @default.
• W2213825066 cites W2063897278 @default.
• W2213825066 cites W2071102168 @default.
• W2213825066 cites W2072366248 @default.
• W2213825066 cites W2072857193 @default.
• W2213825066 cites W2077156283 @default.
• W2213825066 cites W2081846733 @default.
• W2213825066 cites W2083620029 @default.
• W2213825066 cites W2087846995 @default.
• W2213825066 cites W2089331480 @default.
• W2213825066 cites W2090415455 @default.
• W2213825066 cites W2092706511 @default.
• W2213825066 cites W2097902994 @default.
• W2213825066 cites W2102902733 @default.
• W2213825066 cites W2103725795 @default.
• W2213825066 cites W2104267463 @default.
• W2213825066 cites W2105835695 @default.
• W2213825066 cites W2106704490 @default.
• W2213825066 cites W2111527861 @default.
• W2213825066 cites W2116916154 @default.
• W2213825066 cites W2120919608 @default.
• W2213825066 cites W2129388681 @default.
• W2213825066 cites W2133220184 @default.
• W2213825066 cites W2134068468 @default.
• W2213825066 cites W2138993728 @default.
• W2213825066 cites W2143091276 @default.
• W2213825066 cites W2143761147 @default.
• W2213825066 cites W2146244930 @default.
• W2213825066 cites W2152225923 @default.
• W2213825066 cites W2155921993 @default.
• W2213825066 cites W2159152993 @default.
• W2213825066 cites W2164186503 @default.
• W2213825066 cites W2164305255 @default.
• W2213825066 cites W2168869871 @default.
• W2213825066 cites W2317179256 @default.
• W2213825066 doi "https://doi.org/10.1016/j.bbamem.2015.12.019" @default.
• W2213825066 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26706098" @default.
• W2213825066 hasPublicationYear "2016" @default.
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