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- W2214273722 abstract "The structural protein P32 gene of the goat poxvirus in the recombinant plasmid pMD18-P32 was subcloned into the Pichia pastoris expression vector pPIC9K.The resultant recombinant plasmid pPIC9K-P32 was transformed into P.pastoris GS115 by electroporation.The multi-copy recombinant P.pastoris strains were screened by G418 and induced by methanol.The expressed product was analyzed by SDS-PAGE and Western blotting tests.The results indicated that the aim protein was expressed in P.pastoris,and had an immunological activity,the protein could be recognized by anti-serum against goat poxvirus." @default.
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- W2214273722 date "2009-01-01" @default.
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- W2214273722 title "Construction and expression of recombinant antigen P32 gene of goat poxvirus" @default.
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