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- W221454761 abstract "Abstract Genetic-complementation experiments have shown previously that the mutation p rm116 , which maps near the right operator in phage lambda, inactivates the p rm promoter but not the p re promoter for c I (repressor) gene expression. Direct assays for repressor synthesis in vivo confirm these conclusions: first, λp rm116 does not synthesize repressor after infection of an immune lysogen, when p rm should normally be active; second, activation of p re by c II and c III proteins after infection of nonlysogenic cells is not affected by the p rm116 mutation. Unexpectedly, however, λp rm116 does not shut off repressor synthesis at the normal time after infection of a nonlysogen, so that, in fact, repressor levels late in infection with λp rm116 are actually greater than those observed after wild-type infection. Since cro gene product is required to shut off repressor synthesis under these conditions, we speculate that the p rm116 mutation may also alter a cro target site near the right operator." @default.
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- W221454761 date "1975-01-01" @default.
- W221454761 modified "2023-09-24" @default.
- W221454761 title "Repressor synthesis in vivo after infection of E. coli by a prm− mutant of bacteriophage λ" @default.
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- W221454761 doi "https://doi.org/10.1016/0042-6822(75)90391-8" @default.
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