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• W2214745938 startingPage "715" @default.
• W2214745938 abstract "Abstract Widespread evidence supports the existence of an endogenous digitalis-like compound in mammals. We report here the development of a novel enzyme immunoassay for ouabain that, in conjunction with a detailed HPLC study, identifies a ouabain-like compound (OLC) in extracted human plasma. The assay is sensitive—minimum detection limit for OLC 37 pmol/L (11 pmol/L in plasma)—and has a working range (between-assay CV &lt;10%) of 180–10 000 pmol/L (54–3000 pmol/L in plasma). Mean recoveries of ouabain added to plasma ranged from 90% to 100%, and plasma extracts diluted in parallel to the standard curve. Plasma OLC concentrations in 10 healthy volunteers averaged 92 pmol/L (range 55–168), assuming 100% cross-reactivity of OLC in the ouabain assay. HPLC analysis with two distinct chromatographic conditions demonstrated that endogenous human plasma OLC co-eluted with authentic ouabain. The enzyme immunoassay is rapid and easy to perform and will support further investigation of the nature of this controversial endogenous steroid." @default.
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• W2214745938 date "1997-05-01" @default.
• W2214745938 modified "2023-10-13" @default.
• W2214745938 title "Development of enzyme immunoassay for endogenous ouabain-like compound in human plasma" @default.
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• W2214745938 doi "https://doi.org/10.1093/clinchem/43.5.715" @default.
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