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- W2214767553 abstract "Membrane androgen receptors (mAR) are functionally expressed in a variety of tumor-cells including the breast tumor-cell line MCF-7. They are specifically activated by testosterone albumin conjugates (TAC). The mAR sensitive signaling includes activation of Ras-related C3 botulinum toxin substrate 1 (Rac1) and reorganization of the actin filament network. Signaling of tumor-cells may further involve up-regulation of pore forming Ca2+ channel protein Orai1, which accomplishes store operated Ca2+ entry (SOCE). This study explored the regulation of Orai1 abundance and SOCE by mAR. Actin filaments were visualized utilizing confocal microscopy, Rac1 activity using GST-GBD assay, Orai1 transcript levels by RT-PCR and total protein abundance by western blotting, Orai1 abundance at the cell surface by confocal microscopy and FACS-analysis, cytosolic Ca2+ activity ([Ca2+]i) utilizing Fura-2-fluorescence, and SOCE from increase of [Ca2+]i following readdition of Ca2+ after store depletion with thapsigargin (1 μM). TAC treatment of MCF-7 cells was followed by Rac1 activation, actin polymerization, transient increase of Orai1transcript levels and protein abundance, and transient increase of SOCE. The transient increase of Orai1 protein abundance was abrogated by Rac1 inhibitor NSC23766 (50 μM) and by prevention of actin reorganization with cytochalasin B (1 μM). mAR sensitive Rac1 activation and actin reorganization contribute to the regulation of Orai1 protein abundance and SOCE." @default.
- W2214767553 created "2016-06-24" @default.
- W2214767553 creator A5014229340 @default.
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- W2214767553 creator A5084208566 @default.
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- W2214767553 date "2015-12-01" @default.
- W2214767553 modified "2023-09-26" @default.
- W2214767553 title "Up-regulation of Orai1 expression and store operated Ca2+ entry following activation of membrane androgen receptors in MCF-7 breast tumor cells" @default.
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- W2214767553 cites W1531851959 @default.
- W2214767553 cites W1560014945 @default.
- W2214767553 cites W1582135689 @default.
- W2214767553 cites W1599277155 @default.
- W2214767553 cites W1607531254 @default.
- W2214767553 cites W1784820495 @default.
- W2214767553 cites W1860041252 @default.
- W2214767553 cites W1920988707 @default.
- W2214767553 cites W1963703355 @default.
- W2214767553 cites W1965519224 @default.
- W2214767553 cites W1965722585 @default.
- W2214767553 cites W1974968903 @default.
- W2214767553 cites W1984278612 @default.
- W2214767553 cites W1991920041 @default.
- W2214767553 cites W1997267832 @default.
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- W2214767553 cites W2005918516 @default.
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- W2214767553 cites W2013896822 @default.
- W2214767553 cites W2015426350 @default.
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- W2214767553 cites W2022548775 @default.
- W2214767553 cites W2024432827 @default.
- W2214767553 cites W2026572984 @default.
- W2214767553 cites W2029301182 @default.
- W2214767553 cites W2036145275 @default.
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- W2214767553 cites W2047128497 @default.
- W2214767553 cites W2050350148 @default.
- W2214767553 cites W2053907473 @default.
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- W2214767553 cites W2064746517 @default.
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- W2214767553 cites W2080145523 @default.
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- W2214767553 cites W2087806432 @default.
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- W2214767553 doi "https://doi.org/10.1186/s12885-015-2014-2" @default.
- W2214767553 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4687293" @default.
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- W2214767553 hasPublicationYear "2015" @default.
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