FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2214882132> ?p ?o ?g. }

• W2214882132 abstract "Protein-protein interactions (PPIs) are key molecular events to biology. However, it remains a challenge to visualize PPIs with sufficient resolution and sensitivity in cells because the resolution of conventional light microscopy is diffraction-limited to ~250 nm. By combining bimolecular fluorescence complementation (BiFC) with photoactivated localization microscopy (PALM), PPIs can be visualized in cells with single molecule sensitivity and nanometer spatial resolution. BiFC is a commonly used technique for visualizing PPIs with fluorescence contrast, which involves splitting of a fluorescent protein into two non-fluorescent fragments. PALM is a recent superresolution microscopy technique for imaging biological samples at the nanometer and single molecule scales, which uses phototransformable fluorescent probes such as photoactivatable fluorescent proteins (PA-FPs). BiFC-PALM was demonstrated by splitting PAmCherry1, a PA-FP compatible with PALM, for its monomeric nature, good single molecule brightness, high contrast ratio, and utility for stoichiometry measurements. When split between amino acids 159 and 160, PAmCherry1 can be made into a BiFC probe that reconstitutes efficiently at 37 °C with high specificity to PPIs and low non-specific reconstitution. Ras-Raf interaction is used as an example to show how BiFC-PALM helps to probe interactions at the nanometer scale and with single molecule resolution. Their diffusion can also be tracked in live cells using single molecule tracking (smt-) PALM. In this protocol, factors to consider when designing the fusion proteins for BiFC-PALM are discussed, sample preparation, image acquisition, and data analysis steps are explained, and a few exemplary results are showcased. Providing high spatial resolution, specificity, and sensitivity, BiFC-PALM is a useful tool for studying PPIs in intact biological samples." @default.
• W2214882132 created "2016-06-24" @default.
• W2214882132 creator A5016202744 @default.
• W2214882132 creator A5022186474 @default.
• W2214882132 creator A5023067564 @default.
• W2214882132 creator A5061329295 @default.
• W2214882132 date "2015-12-22" @default.
• W2214882132 modified "2023-10-13" @default.
• W2214882132 title "Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)" @default.
• W2214882132 cites W1519019441 @default.
• W2214882132 cites W1525412307 @default.
• W2214882132 cites W1598771428 @default.
• W2214882132 cites W1965169361 @default.
• W2214882132 cites W1972549673 @default.
• W2214882132 cites W1972919132 @default.
• W2214882132 cites W1987195331 @default.
• W2214882132 cites W1987660915 @default.
• W2214882132 cites W2011423264 @default.
• W2214882132 cites W2021882008 @default.
• W2214882132 cites W2026012812 @default.
• W2214882132 cites W2030632688 @default.
• W2214882132 cites W2037611736 @default.
• W2214882132 cites W2043409309 @default.
• W2214882132 cites W2049029186 @default.
• W2214882132 cites W2054455698 @default.
• W2214882132 cites W2075242130 @default.
• W2214882132 cites W2076115100 @default.
• W2214882132 cites W2088064651 @default.
• W2214882132 cites W2091804920 @default.
• W2214882132 cites W2101336703 @default.
• W2214882132 cites W2103706175 @default.
• W2214882132 cites W2118212125 @default.
• W2214882132 cites W2136558879 @default.
• W2214882132 cites W2138865250 @default.
• W2214882132 cites W2167892316 @default.
• W2214882132 cites W2169658668 @default.
• W2214882132 cites W2170725518 @default.
• W2214882132 cites W2974780560 @default.
• W2214882132 doi "https://doi.org/10.3791/53154" @default.
• W2214882132 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4758764" @default.
• W2214882132 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26779930" @default.
• W2214882132 hasPublicationYear "2015" @default.
• W2214882132 type Work @default.
• W2214882132 sameAs 2214882132 @default.
• W2214882132 citedByCount "11" @default.
• W2214882132 countsByYear W22148821322017 @default.
• W2214882132 countsByYear W22148821322018 @default.
• W2214882132 countsByYear W22148821322019 @default.
• W2214882132 countsByYear W22148821322020 @default.
• W2214882132 countsByYear W22148821322022 @default.
• W2214882132 countsByYear W22148821322023 @default.
• W2214882132 crossrefType "journal-article" @default.
• W2214882132 hasAuthorship W2214882132A5016202744 @default.
• W2214882132 hasAuthorship W2214882132A5022186474 @default.
• W2214882132 hasAuthorship W2214882132A5023067564 @default.
• W2214882132 hasAuthorship W2214882132A5061329295 @default.
• W2214882132 hasBestOaLocation W22148821322 @default.
• W2214882132 hasConcept C116919411 @default.
• W2214882132 hasConcept C120665830 @default.
• W2214882132 hasConcept C121332964 @default.
• W2214882132 hasConcept C123243835 @default.
• W2214882132 hasConcept C12554922 @default.
• W2214882132 hasConcept C138268822 @default.
• W2214882132 hasConcept C147080431 @default.
• W2214882132 hasConcept C154945302 @default.
• W2214882132 hasConcept C166936260 @default.
• W2214882132 hasConcept C169274487 @default.
• W2214882132 hasConcept C171250308 @default.
• W2214882132 hasConcept C185592680 @default.
• W2214882132 hasConcept C192562407 @default.
• W2214882132 hasConcept C41008148 @default.
• W2214882132 hasConcept C86803240 @default.
• W2214882132 hasConcept C91881484 @default.
• W2214882132 hasConceptScore W2214882132C116919411 @default.
• W2214882132 hasConceptScore W2214882132C120665830 @default.
• W2214882132 hasConceptScore W2214882132C121332964 @default.
• W2214882132 hasConceptScore W2214882132C123243835 @default.
• W2214882132 hasConceptScore W2214882132C12554922 @default.
• W2214882132 hasConceptScore W2214882132C138268822 @default.
• W2214882132 hasConceptScore W2214882132C147080431 @default.
• W2214882132 hasConceptScore W2214882132C154945302 @default.
• W2214882132 hasConceptScore W2214882132C166936260 @default.
• W2214882132 hasConceptScore W2214882132C169274487 @default.
• W2214882132 hasConceptScore W2214882132C171250308 @default.
• W2214882132 hasConceptScore W2214882132C185592680 @default.
• W2214882132 hasConceptScore W2214882132C192562407 @default.
• W2214882132 hasConceptScore W2214882132C41008148 @default.
• W2214882132 hasConceptScore W2214882132C86803240 @default.
• W2214882132 hasConceptScore W2214882132C91881484 @default.
• W2214882132 hasIssue "106" @default.
• W2214882132 hasLocation W22148821321 @default.
• W2214882132 hasLocation W22148821322 @default.
• W2214882132 hasLocation W22148821323 @default.
• W2214882132 hasLocation W22148821324 @default.
• W2214882132 hasOpenAccess W2214882132 @default.
• W2214882132 hasPrimaryLocation W22148821321 @default.
• W2214882132 hasRelatedWork W2094643072 @default.
• W2214882132 hasRelatedWork W2122359996 @default.
• W2214882132 hasRelatedWork W2156311466 @default.
• W2214882132 hasRelatedWork W2406124160 @default.

• next