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- W2214982481 abstract "The acid phosphatases in Piriformospora indica and Sebacina vermifera sensu were similar in their molecular mass. The raised antibody against acid phosphatase (ACPase) of P. indica showed maximum ELISA reading with S. vermifera sensu supporting strong relationship between these two fungi. The immunoblot analysis showed the strong reactivity of antiserum with S. vermifera sensu. The antiserum blotted the bands of 66 kDa in S. vermifera separated in denatured—PAGE at equidistance with P. indica. The enzyme assay zymogram of non-denatured-ACPase showed parallel bands at the same height in native PAGE. The antiserum also localized the enzyme in S. vermifera by immunofluorescence technique, showing strong relationship of this fungus with P. indica. The immunogold labeling of antiserum from P. indica precisely localized the enzyme in cytoplasm and vacuoles of S. vermifera supporting the strong immunological link between these two fungi. Two-dimensional map of crude protein of these two fungi showed some differences in minor proteins. P. indica and S. vermifera sensu belonging to the same taxonomic group show similar morphology, isozyme characteristics, and various functions as well. However, they show distinct genetic variation based on the random amplified polymorphic DNA (RAPD) analysis." @default.
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- W2214982481 date "2013-01-01" @default.
- W2214982481 modified "2023-09-27" @default.
- W2214982481 title "Similarity Between Piriformospora indica and Sebacina vermifera Sensu Members of the Order Sebacinales Based on Immunological Techniques" @default.
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