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• W2217504676 endingPage "e0140591" @default.
• W2217504676 startingPage "e0140591" @default.
• W2217504676 abstract "Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants. However, the roles played by this protein in salt stress responses in wheat (Triticum aestivum) remain obscure. In this study, three TaCRT genes were identified in wheat and named TaCRT1, TaCRT2 and TaCRT3-1 based on their sequence characteristics and their high homology to other known CRT genes. Quantitative real-time PCR expression data revealed that these three genes exhibit different expression patterns in different tissues and are strongly induced under salt stress in wheat. The calcium-binding properties of the purified recombinant TaCRT1 protein were determined using a PIPES/Arsenazo III analysis. TaCRT1 gene overexpression in Nicotiana tabacum decreased salt stress damage in transgenic tobacco plants. Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions. Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants." @default.
• W2217504676 created "2016-06-24" @default.
• W2217504676 creator A5001716676 @default.
• W2217504676 creator A5017681571 @default.
• W2217504676 creator A5037092954 @default.
• W2217504676 creator A5049157776 @default.
• W2217504676 creator A5056000409 @default.
• W2217504676 creator A5061693687 @default.
• W2217504676 creator A5066937441 @default.
• W2217504676 creator A5068457792 @default.
• W2217504676 date "2015-10-15" @default.
• W2217504676 modified "2023-10-06" @default.
• W2217504676 title "Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco" @default.
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