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- W2217757517 abstract "Objective To Construction of P and NP genes eukaryotic expression vectors of Newcastle Disease Virus LaSota strain,study its reverse genetics and functional genome of NDV. Methods P, NP genes were amplified and cloned into pGEM-T easy vector and then subcloned into pcDNA3.1 (+) expression vector respectively, the recombinant plasmids were named pcDNA3.1(+)-P and pcDNA3.1 (+)-NP, Recombinant plasmids were transfected into 293 and BHK-21 cells respectively and were detected using IE and Western blot analysis. Results Expression of P, NP genes were detected and confirmed by the IE and WB analysis. Conclusion The recombinant eukaryotic plasmids pcDNA3.1 (+)-P, pcDNA3.1 (+)-NP were expressed in 293 and BHK-21 cells successfully. This research may be helpful for further study of reverse genetics and functional genome of NDV.Key words: Newcastle disease virus ; Viral protein; Reverse genetics manipulation system" @default.
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- W2217757517 date "2010-02-01" @default.
- W2217757517 modified "2023-09-28" @default.
- W2217757517 title "Transient expression and identification of gene P and NP of NDV LaSota strain in two different cells" @default.
- W2217757517 doi "https://doi.org/10.3760/cma.j.issn.1003-9279.2010.01.022" @default.
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