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- W2218162643 abstract "To interpret the functions of monoamines, it is unquestionably necessary to be able to localize accurately their tissue storage sites. The available histochemical methods have one feature in common: their sensitivity is low and permits the demonstration of monoamines only in cells that store them in high concentrations, for example, adrenal medullary cells and enterochromaffin cells. A more promising degree of progress was obtained with the finding that certain catecholamines, included in a dry protein film, are transformed into products with an intense green to yellow–green fluorescence on exposure to formaldehyde gas. Investigations on the chemical nature and specificity of this reaction have been performed with model systems. The primary catecholamines react readily with formaldehyde and are transformed into intensely green to sometimes yellow–green fluorescent compounds within 1-h treatment. The same fluorescence can be obtained from secondary catecholamines, such as A, but only if the formaldehyde treatment is prolonged to 2–3 h. Tryptamines, such as 5-hydroxytryptamine (5-HT), react as readily as the primary catecholamines. The fluorophore of 5-HT, however, emits an intense yellow fluorescence. In this way, the method offers a possibility to differentiate between primary and secondary catecholamines as well as between catecholamines and tryptamines." @default.
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- W2218162643 date "1964-01-01" @default.
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- W2218162643 title "Cellular Localization of Monoamines" @default.
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- W2218162643 doi "https://doi.org/10.1016/s0079-6123(08)60111-9" @default.
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