FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2218432009> ?p ?o ?g. }

• W2218432009 endingPage "4994" @default.
• W2218432009 startingPage "4994" @default.
• W2218432009 abstract "AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA4994 Background: Tobacco smoke exposure is the major cause of lung cancer. Long term heavy smokers retain an elevated lung cancer risk even years after smoking cessation. Former smokers now account for nearly half of the newly diagnosed lung cancer cases. The mechanism for lung cancer development despite smoking cessation is still poorly understood. In this study, using serial analysis of gene expression (SAGE), we compare the transcriptomes of bronchial cells obtained from current, former and never smokers to identify genes whose expression were reversible and irreversible upon smoking cessation.Objectives: (1) To define the transcriptomes of current, former and never smokers. (2) To identify genes which are dysregulated with active smoking. (3) To identify changes that are irreversible upon smoking cessation.DESIGN, MATERIALS AND METHODS: Twenty-four bronchial epithelial specimens from the peripheral airways obtained during fluorescence bronchoscopy were profiled using SAGE. This sample set was comprised of 8 current smokers, 12 former smokers and 4 never smokers. Transcriptomes for current, former and never smokers were defined using a tag count of ≥2 across all libraries in each set. Genes differentially expressed were identified using the Mann Whitney U test. Select genes were validated in a secondary cohort of 9 current smokers, 7 former smokers and 6 never smokers using quantitative RT-PCR.Results: 3,111,471 SAGE sequence tags representing over 110,000 potentially unique transcripts were generated, representing one of the largest human SAGE studies to date. We identified 1733 genes to be constitutively expressed in current, former and never smoker transcriptomes. Reversible gene expression changes upon smoking cessation were identified, mainly falling into xenobiotic / nucleotide metabolism and muco-ciliary response and clearance. Significantly, altered expression of calcium binding tyrosine-(Y)-phosphorylation regulated (CABYR) and ectonucleoside triphosphate diphosphohydrolase 8 (ENTPD8) is reversible upon smoking cessation, neither of which have previously been shown to be expressed in the bronchial epithelium. Conversely, glycogen synthase kinase 3 beta (GSK3B) , mucin 5AC (MUC5AC) demonstrated irreversible expression changes upon smoking cessation. All of these gene expression profiles were validated in a second cohort of samples.Conclusions: Irreversible changes in expression of genes associated with inflammatory pathways and DNA repair may account for substantial DNA damage, and increased susceptibility to lung cancer despite smoking cessation. This work was supported by Genome BC / Genome Canada." @default.
• W2218432009 created "2016-06-24" @default.
• W2218432009 creator A5001009374 @default.
• W2218432009 creator A5013908616 @default.
• W2218432009 creator A5015216658 @default.
• W2218432009 creator A5015304965 @default.
• W2218432009 creator A5025947047 @default.
• W2218432009 creator A5031888358 @default.
• W2218432009 date "2007-05-01" @default.
• W2218432009 modified "2023-09-27" @default.
• W2218432009 title "Effect of active smoking on the bronchial epithelium transcriptome" @default.
• W2218432009 hasPublicationYear "2007" @default.
• W2218432009 type Work @default.
• W2218432009 sameAs 2218432009 @default.
• W2218432009 citedByCount "0" @default.
• W2218432009 crossrefType "journal-article" @default.
• W2218432009 hasAuthorship W2218432009A5001009374 @default.
• W2218432009 hasAuthorship W2218432009A5013908616 @default.
• W2218432009 hasAuthorship W2218432009A5015216658 @default.
• W2218432009 hasAuthorship W2218432009A5015304965 @default.
• W2218432009 hasAuthorship W2218432009A5025947047 @default.
• W2218432009 hasAuthorship W2218432009A5031888358 @default.
• W2218432009 hasConcept C104317684 @default.
• W2218432009 hasConcept C121608353 @default.
• W2218432009 hasConcept C126322002 @default.
• W2218432009 hasConcept C142724271 @default.
• W2218432009 hasConcept C143998085 @default.
• W2218432009 hasConcept C150194340 @default.
• W2218432009 hasConcept C162317418 @default.
• W2218432009 hasConcept C2776256026 @default.
• W2218432009 hasConcept C2777405583 @default.
• W2218432009 hasConcept C2777714996 @default.
• W2218432009 hasConcept C2777843972 @default.
• W2218432009 hasConcept C2778355491 @default.
• W2218432009 hasConcept C54355233 @default.
• W2218432009 hasConcept C71924100 @default.
• W2218432009 hasConcept C72563966 @default.
• W2218432009 hasConcept C86803240 @default.
• W2218432009 hasConcept C99454951 @default.
• W2218432009 hasConceptScore W2218432009C104317684 @default.
• W2218432009 hasConceptScore W2218432009C121608353 @default.
• W2218432009 hasConceptScore W2218432009C126322002 @default.
• W2218432009 hasConceptScore W2218432009C142724271 @default.
• W2218432009 hasConceptScore W2218432009C143998085 @default.
• W2218432009 hasConceptScore W2218432009C150194340 @default.
• W2218432009 hasConceptScore W2218432009C162317418 @default.
• W2218432009 hasConceptScore W2218432009C2776256026 @default.
• W2218432009 hasConceptScore W2218432009C2777405583 @default.
• W2218432009 hasConceptScore W2218432009C2777714996 @default.
• W2218432009 hasConceptScore W2218432009C2777843972 @default.
• W2218432009 hasConceptScore W2218432009C2778355491 @default.
• W2218432009 hasConceptScore W2218432009C54355233 @default.
• W2218432009 hasConceptScore W2218432009C71924100 @default.
• W2218432009 hasConceptScore W2218432009C72563966 @default.
• W2218432009 hasConceptScore W2218432009C86803240 @default.
• W2218432009 hasConceptScore W2218432009C99454951 @default.
• W2218432009 hasLocation W22184320091 @default.
• W2218432009 hasOpenAccess W2218432009 @default.
• W2218432009 hasPrimaryLocation W22184320091 @default.
• W2218432009 hasRelatedWork W1123879001 @default.
• W2218432009 hasRelatedWork W1972594262 @default.
• W2218432009 hasRelatedWork W1986702393 @default.
• W2218432009 hasRelatedWork W1994980537 @default.
• W2218432009 hasRelatedWork W2001874241 @default.
• W2218432009 hasRelatedWork W2023263432 @default.
• W2218432009 hasRelatedWork W2032791994 @default.
• W2218432009 hasRelatedWork W2145663969 @default.
• W2218432009 hasRelatedWork W2145746534 @default.
• W2218432009 hasRelatedWork W2146728395 @default.
• W2218432009 hasRelatedWork W2157752105 @default.
• W2218432009 hasRelatedWork W2181525124 @default.
• W2218432009 hasRelatedWork W2775170117 @default.
• W2218432009 hasRelatedWork W2885884316 @default.
• W2218432009 hasRelatedWork W2887957697 @default.
• W2218432009 hasRelatedWork W2950298946 @default.
• W2218432009 hasRelatedWork W2973160842 @default.
• W2218432009 hasRelatedWork W3044858697 @default.
• W2218432009 hasRelatedWork W3199142017 @default.
• W2218432009 hasRelatedWork W566036147 @default.
• W2218432009 hasVolume "67" @default.
• W2218432009 isParatext "false" @default.
• W2218432009 isRetracted "false" @default.
• W2218432009 magId "2218432009" @default.
• W2218432009 workType "article" @default.