Matches in SemOpenAlex for { <https://semopenalex.org/work/W2218508717> ?p ?o ?g. }
- W2218508717 endingPage "46" @default.
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- W2218508717 abstract "We developed a method for extracting raft-like, liquid-ordered membranes from the particulate fraction prepared from porcine trachealis smooth muscle. This fraction, which contains most of the plasma membrane in this tissue, was homogenized in the presence of cold 0.5% Triton X-100. After centrifugation, membranes containing high contents of sphingomyelin (SM) and cholesterol and low phosphatidylcholine (PC) contents remained in the pellet. Thirty-five millimolar octyl glucoside (OG) extracted 75% of these membranes from the Triton X-100-resistant pellet. These membranes had low buoyant densities and accounted for 28% of the particulate fraction lipid. Their lipid composition, 22% SM, 60% cholesterol, 11% phosphatidylethanolamine, 8% PC, <1% phosphatidylinositol, and coisolation with 5′-nucleotidase and caveolin-1 suggest that they are liquid-ordered membranes. We compared characteristics of OG and Triton X-100 extractions of the particulate fraction. In contrast to Triton X-100 extractions, membranes released from the particulate fraction by OG were mainly collected in low buoyant fractions at densities ranging from 1.05 to 1.11 g/ml and had phospholipid and cholesterol contents consistent with a mixture of liquid-ordered and liquid-disordered membranes.Thus, OG extraction of apparent liquid-ordered membranes from Triton X-100-resistant pellets was not due to selective extraction of these membranes. Low buoyant density appears not to be unique for liquid-ordered membranes." @default.
- W2218508717 created "2016-06-24" @default.
- W2218508717 creator A5025713708 @default.
- W2218508717 creator A5076443395 @default.
- W2218508717 date "1994-01-01" @default.
- W2218508717 modified "2023-10-09" @default.
- W2218508717 title "Plasma Membrane Phospholipid Asymmetry and Its Maintenance: The Human Erythrocyte as a Model" @default.
- W2218508717 cites W1486313261 @default.
- W2218508717 cites W1491471718 @default.
- W2218508717 cites W1497435349 @default.
- W2218508717 cites W1497822816 @default.
- W2218508717 cites W1575458839 @default.
- W2218508717 cites W1578213988 @default.
- W2218508717 cites W1580615597 @default.
- W2218508717 cites W1608391891 @default.
- W2218508717 cites W166250711 @default.
- W2218508717 cites W1900430587 @default.
- W2218508717 cites W1965331592 @default.
- W2218508717 cites W1965721525 @default.
- W2218508717 cites W1966038156 @default.
- W2218508717 cites W1966388528 @default.
- W2218508717 cites W1967712318 @default.
- W2218508717 cites W1968474231 @default.
- W2218508717 cites W1969013901 @default.
- W2218508717 cites W1969383350 @default.
- W2218508717 cites W1974255968 @default.
- W2218508717 cites W1977698345 @default.
- W2218508717 cites W1977986845 @default.
- W2218508717 cites W1978255448 @default.
- W2218508717 cites W1978577589 @default.
- W2218508717 cites W1979233174 @default.
- W2218508717 cites W1980224025 @default.
- W2218508717 cites W1981472108 @default.
- W2218508717 cites W1981776612 @default.
- W2218508717 cites W1982805342 @default.
- W2218508717 cites W1983378847 @default.
- W2218508717 cites W1985101459 @default.
- W2218508717 cites W1985121273 @default.
- W2218508717 cites W1986444104 @default.
- W2218508717 cites W1986566264 @default.
- W2218508717 cites W1986962123 @default.
- W2218508717 cites W1987410604 @default.
- W2218508717 cites W1988491028 @default.
- W2218508717 cites W1989518167 @default.
- W2218508717 cites W1990787887 @default.
- W2218508717 cites W1992052771 @default.
- W2218508717 cites W1992928034 @default.
- W2218508717 cites W1993733633 @default.
- W2218508717 cites W1995532798 @default.
- W2218508717 cites W1999430913 @default.
- W2218508717 cites W2000266304 @default.
- W2218508717 cites W2000602772 @default.
- W2218508717 cites W2000820013 @default.
- W2218508717 cites W2001865088 @default.
- W2218508717 cites W2002604567 @default.
- W2218508717 cites W2003331008 @default.
- W2218508717 cites W2004336149 @default.
- W2218508717 cites W2004656105 @default.
- W2218508717 cites W2005502942 @default.
- W2218508717 cites W2005859080 @default.
- W2218508717 cites W2006191177 @default.
- W2218508717 cites W2006302624 @default.
- W2218508717 cites W2006506188 @default.
- W2218508717 cites W2006831581 @default.
- W2218508717 cites W2008228632 @default.
- W2218508717 cites W2008586837 @default.
- W2218508717 cites W2010550024 @default.
- W2218508717 cites W2011523704 @default.
- W2218508717 cites W2013615291 @default.
- W2218508717 cites W2014279568 @default.
- W2218508717 cites W2014826463 @default.
- W2218508717 cites W2015668441 @default.
- W2218508717 cites W2017152618 @default.
- W2218508717 cites W2018349666 @default.
- W2218508717 cites W2018641971 @default.
- W2218508717 cites W2019649931 @default.
- W2218508717 cites W2020627996 @default.
- W2218508717 cites W2021105020 @default.
- W2218508717 cites W2021360602 @default.
- W2218508717 cites W2021429496 @default.
- W2218508717 cites W2022843125 @default.
- W2218508717 cites W2023773482 @default.
- W2218508717 cites W2024302733 @default.
- W2218508717 cites W2025792213 @default.
- W2218508717 cites W2026012089 @default.
- W2218508717 cites W2026462635 @default.
- W2218508717 cites W2027214066 @default.
- W2218508717 cites W2028744351 @default.
- W2218508717 cites W2029721348 @default.
- W2218508717 cites W2030001254 @default.
- W2218508717 cites W2030747544 @default.
- W2218508717 cites W2032358766 @default.
- W2218508717 cites W2032682369 @default.
- W2218508717 cites W2035591072 @default.
- W2218508717 cites W2036452446 @default.
- W2218508717 cites W2037124362 @default.
- W2218508717 cites W2037151582 @default.
- W2218508717 cites W2037344193 @default.