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• W2219017964 abstract "Objective To construct eukaryotic cell expression plasmid of pmCherry-C2 fusion protein and analysis its expression and intracellular localization in NIH3T3 cell. Methods Septin 8 coding region was amplified by PCR from human glioma cells cDNA library and subcloned into pmCherry-C2 vector. After comfirmed by PCR, enzyme digestion and DNA sequencing,the construct was then transfected into NIH3T3 cells and analyzed by fluorescence microscopy. Results pmCherry-C2/septin 8 fusion protein was highly expressed in NIH3T3 cell. Fluorescence microscope analysis revealed that the fusion protein is mainly distributed in the cytoplasm. Conclusion The pmCherry-C2 / septin 8 fusion protein plasmid has been successfully constructed and expressed in NIH3T3 cell, which laid the foundation for the functional analysis of septin 8 in the future." @default.
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• W2219017964 date "2010-01-01" @default.
• W2219017964 modified "2023-09-24" @default.
• W2219017964 title "Construction and Expression of Setpin 8-red Fluorescent Fusion Protein Vector" @default.
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