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- W2219224590 abstract "We have developed a nanopore sensing approach for the selective detection of 8-oxo-2′-deoxyguanosine (8-oxoG) in single-stranded DNA. First, 1,12-dodecanediamine is coupled with 8-oxoG-containing DNA molecules in high yield which leaves a free amine group for subsequent attaching of an adamantane moiety. After incubation with cucurbit[7]uril, the host–guest complex-modified DNA hybrid is translocated through an α-hemolysin nanopore. Highly characteristic events can be recorded and used to quantify the 8-oxoG-DNA content in a DNA mixture. Compared with the existing methods, this study provides a reliable, quick, and low-cost approach for the detection of 8-oxoG site in single-stranded DNA at the single-molecule level, particularly suitable for high-throughput screening of a massive number of samples." @default.
- W2219224590 created "2016-06-24" @default.
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- W2219224590 date "2015-12-29" @default.
- W2219224590 modified "2023-10-18" @default.
- W2219224590 title "Selective Detection of 8-Oxo-2′-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach" @default.
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- W2219224590 doi "https://doi.org/10.1021/acs.analchem.5b04102" @default.
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