Matches in SemOpenAlex for { <https://semopenalex.org/work/W22195133> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W22195133 endingPage "19" @default.
- W22195133 startingPage "18" @default.
- W22195133 abstract "Quantitative real-time reverse-transcription PCR (RT-qPCR) is presently the method of choice for validating gene expression results from high-density microarrays. However, the low throughput of traditional gene-by-gene RT-qPCR makes this process labor intensive and time consuming. To accelerate this laborious task, the SuperArray RT²Profiler PCR Array combines SYBR Green–based real-time RT-qPCR technology with a multi-gene array plate format to simultaneously analyze a panel of genes related to a specific disease or biological pathway.Each assay on the PCR array plate has been experimentally validated to insure gene-specific amplification. The reliability and reproducibility of the RT²Profiler PCR Array have been demonstrated by DNA sequencing and intra/inter-laboratory reproducibility comparisons. The DNA sequencing demonstrated 100% of the PCR products amplified from the correct target genes. In one laboratory, individual PCR assays produced a standard deviation of 0.24 cycles and a coefficient of variance of 0.92% in technical replicates. The correlation coefficient for Ct values between replicate runs was 0.997 and for fold changes (ΔΔCt) across thermocyclers was 0.976. Comparisons between two different laboratories using different thermocyclers showed correlation coefficients of 0.972 and 0.976 for ΔCt and ΔΔCt, respectively. Each PCR array also includes stringent controls to monitor RNA quality by assessing reverse transcription efficiency and genomic DNA contamination to ensure the reliability of the PCR array data.A practical application for the PCR array was demonstrated by identifying human pancreatic tumor–associated genes using the Cancer PathwayFinder RT²Profiler PCR Array. Results showed 23 genes exhibiting a statistically significant threefold or greater change in expression between a human pancreatic tumor and normal pancreas, including many genes previously linked to pancreatic cancer. Hence, the RT2 Profiler PCR Array system offers a simple, reliable, and convenient tool for multi-gene profiling and microarray data validation." @default.
- W22195133 created "2016-06-24" @default.
- W22195133 creator A5007469636 @default.
- W22195133 creator A5017346491 @default.
- W22195133 creator A5025943107 @default.
- W22195133 creator A5027521270 @default.
- W22195133 creator A5028595213 @default.
- W22195133 creator A5047219291 @default.
- W22195133 creator A5053928863 @default.
- W22195133 creator A5056878522 @default.
- W22195133 creator A5068970143 @default.
- W22195133 creator A5082479281 @default.
- W22195133 creator A5088510694 @default.
- W22195133 creator A5090661259 @default.
- W22195133 date "2007-02-01" @default.
- W22195133 modified "2023-10-13" @default.
- W22195133 title "P53-M Real-Time PCR Array for Multi-Gene Expression Profiling and Microarray Data Validation: RT2 Profiler PCR Array." @default.
- W22195133 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2291860" @default.
- W22195133 hasPublicationYear "2007" @default.
- W22195133 type Work @default.
- W22195133 sameAs 22195133 @default.
- W22195133 citedByCount "0" @default.
- W22195133 crossrefType "journal-article" @default.
- W22195133 hasAuthorship W22195133A5007469636 @default.
- W22195133 hasAuthorship W22195133A5017346491 @default.
- W22195133 hasAuthorship W22195133A5025943107 @default.
- W22195133 hasAuthorship W22195133A5027521270 @default.
- W22195133 hasAuthorship W22195133A5028595213 @default.
- W22195133 hasAuthorship W22195133A5047219291 @default.
- W22195133 hasAuthorship W22195133A5053928863 @default.
- W22195133 hasAuthorship W22195133A5056878522 @default.
- W22195133 hasAuthorship W22195133A5068970143 @default.
- W22195133 hasAuthorship W22195133A5082479281 @default.
- W22195133 hasAuthorship W22195133A5088510694 @default.
- W22195133 hasAuthorship W22195133A5090661259 @default.
- W22195133 hasConcept C104317684 @default.
- W22195133 hasConcept C105795698 @default.
- W22195133 hasConcept C150194340 @default.
- W22195133 hasConcept C153911025 @default.
- W22195133 hasConcept C18431079 @default.
- W22195133 hasConcept C186836561 @default.
- W22195133 hasConcept C24361400 @default.
- W22195133 hasConcept C2779148672 @default.
- W22195133 hasConcept C2781162219 @default.
- W22195133 hasConcept C33923547 @default.
- W22195133 hasConcept C48023723 @default.
- W22195133 hasConcept C54355233 @default.
- W22195133 hasConcept C70721500 @default.
- W22195133 hasConcept C86803240 @default.
- W22195133 hasConcept C95371953 @default.
- W22195133 hasConcept C9893847 @default.
- W22195133 hasConceptScore W22195133C104317684 @default.
- W22195133 hasConceptScore W22195133C105795698 @default.
- W22195133 hasConceptScore W22195133C150194340 @default.
- W22195133 hasConceptScore W22195133C153911025 @default.
- W22195133 hasConceptScore W22195133C18431079 @default.
- W22195133 hasConceptScore W22195133C186836561 @default.
- W22195133 hasConceptScore W22195133C24361400 @default.
- W22195133 hasConceptScore W22195133C2779148672 @default.
- W22195133 hasConceptScore W22195133C2781162219 @default.
- W22195133 hasConceptScore W22195133C33923547 @default.
- W22195133 hasConceptScore W22195133C48023723 @default.
- W22195133 hasConceptScore W22195133C54355233 @default.
- W22195133 hasConceptScore W22195133C70721500 @default.
- W22195133 hasConceptScore W22195133C86803240 @default.
- W22195133 hasConceptScore W22195133C95371953 @default.
- W22195133 hasConceptScore W22195133C9893847 @default.
- W22195133 hasIssue "1" @default.
- W22195133 hasLocation W221951331 @default.
- W22195133 hasOpenAccess W22195133 @default.
- W22195133 hasPrimaryLocation W221951331 @default.
- W22195133 hasRelatedWork W1742856434 @default.
- W22195133 hasRelatedWork W2033488438 @default.
- W22195133 hasRelatedWork W2040404560 @default.
- W22195133 hasRelatedWork W2049820656 @default.
- W22195133 hasRelatedWork W2061066365 @default.
- W22195133 hasRelatedWork W2082043427 @default.
- W22195133 hasRelatedWork W2111310082 @default.
- W22195133 hasRelatedWork W2115687396 @default.
- W22195133 hasRelatedWork W2133950005 @default.
- W22195133 hasRelatedWork W2181609029 @default.
- W22195133 hasRelatedWork W2182189946 @default.
- W22195133 hasRelatedWork W2384073953 @default.
- W22195133 hasRelatedWork W2425643693 @default.
- W22195133 hasRelatedWork W2426426302 @default.
- W22195133 hasRelatedWork W2757812637 @default.
- W22195133 hasRelatedWork W3038084044 @default.
- W22195133 hasRelatedWork W3102812597 @default.
- W22195133 hasRelatedWork W3167977806 @default.
- W22195133 hasRelatedWork W41381035 @default.
- W22195133 hasRelatedWork W937961548 @default.
- W22195133 hasVolume "18" @default.
- W22195133 isParatext "false" @default.
- W22195133 isRetracted "false" @default.
- W22195133 magId "22195133" @default.
- W22195133 workType "article" @default.