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• W2219952904 abstract "This chapter describes the electron microscopy autoradiography (EM ARG) procedures that are the most widely used for all cytological purposes. It also summarizes the labeling conditions that are considered best to localize newly formed RNA, DNA, or proteins; to date there is no general agreement on the duration of pulse and the time of labeling during the cell cycle. Although a few components of the cell nucleus—for example, the nucleolus—can be identified with routine EM, most of the knowledge on the chemical nature of nuclear structures has been gained through rather complex techniques. The fixatives for EM ARG and EM cytochemistry have to be prepared very carefully and should always be used fresh. They vary according to the technique employed. The methods for nuclear proteins and nucleic acids in general are described in the chapter. The methods are treated according to their claimed specificity, whether assumed or experimentally established. The degree of specificity of each technique is the fundamental problem of cytochemistry. Sensitivity to Pronase hydrolysis, for instance, must be confirmed by [3H] leucine incorporation. Moreover, only the combination of (1) incorporation of the corresponding precursor, (2) specific staining affinity, and (3) sensitivity to the appropriate enzyme can be considered as a full line of evidence." @default.
• W2219952904 created "2016-06-24" @default.
• W2219952904 creator A5035727989 @default.
• W2219952904 creator A5068432911 @default.
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• W2219952904 date "1975-01-01" @default.
• W2219952904 modified "2023-09-24" @default.
• W2219952904 title "[1] Techniques of localization of proteins and nucleoproteins in the cell nucleus by high resolution autoradiography and cytochemistry" @default.
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• W2219952904 doi "https://doi.org/10.1016/s0076-6879(75)40003-9" @default.
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