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- W2220128049 abstract "[Objective] This study aimed to clone the porcine growth hormone gene promoter and determine the core promoter sequences and the cis-acting elements.[Method] Sequence of the 5'flanking region of porcine growth hormone gene was searched out and downloaded from NCBI.According to the targeted sequence,primers were designed and synthesized for the PCR amplification.The 1 882bp(-1 821 bp~+61 bp) fragment was amplified by PCR.Nine promoter fragments with different lengths were obtained by walking deletion and then cloned into luciferase reporter vectors.Relative transcriptional activities of these 5 ' terminal-deleted plasmids in pituitary and non-pituitary cells were determined by transient transfection of the rat pituitary adenoma cell(GH3),porcine iliac endothelium cell(PIEC) and porcrne Kidney-15(PK15) with the constructed dual-luciferase vectors.[Result] Result of DNA sequencing showed that the 1 882 bp fragment of GH 5' promoter was successfully cloned.Nine luciferase reporter gene plasmids were constructed.Dual-Luciferase reporter assay indicated that the promoter inserted into reporter gene vector had very strong cell specificity.[Conclusion] Porcine growth hormone gene specifically expresses in pituitary cells.The minimal promoter of the porcine growth hormone gene is mapped at the region-110~+61 bp.Promoter regions 218~-110 bp and-429 bp~-218 bp contain positive regulatory elements." @default.
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- W2220128049 date "2012-01-01" @default.
- W2220128049 modified "2023-09-23" @default.
- W2220128049 title "Cloning and functional analysis of the porcine growth hormone gene promoter." @default.
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