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- W2220298636 abstract "Publisher Summary This chapter describes aqueous size exclusion chromatography of standard media, fast media, and process media for analysis and purification of macromolecules. The evolution of media covering aqueous and nonaqueous systems on the one hand and microscale and macroscale preparative applications on the other hand has resulted in an arbitrary nomenclature within the field. Thus, the current practice is to refer the separation principle based on solute size as size exclusion chromatography (SEC) whereas the application in aqueous systems is traditionally referred to as gel filtration (GF) and the application in nonaqueous systems is designated gel-permeation chromatography (GPC). Size exclusion is employed for three different applications: for desalting or separation of solutes differing more than a decade in molecular size; for fractionation, where the size differences are smaller—that is, typically a factor of two to five; and for the determination of the molecular weight(s) of a sample." @default.
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- W2220298636 date "1999-01-01" @default.
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- W2220298636 title "Size Exclusion for Analysis and Purification of Aqueous Macromolecules" @default.
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