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• W2220452782 abstract "Yolk sac (YS) is the site of blood-cell production where primitive erythroid cells originate and complete their maturation. YS is a source of precursor cells, however its differentiation potential and suitability for cell therapies are not well described. YS can be a cell source when neovascularization is required. This study characterized YS canine cells, transduced with VEGF, to analyze then using Immunocytochemistry, flow cytometry and real time PCR. Immunocytochemistry: positive expression for CD105, PCNA, VEGF and vWF, flow cytometry for CD105, VEGF, PCNA, OCT-4 and RT-qPCR for VEGF, CD31, CD105, PCNA and FLT – 1, indicating that these cells have characteristics of endothelial progenitor and pluripotency. After transduction, the YS cells changed their morphology and showed endothelial-like cells. We suggest, because of their cell surface phenotype as well as their capacity to differentiate into endothelial-like cells, that canine YS represents a source of cells for neovascularization therapies." @default.
• W2220452782 created "2016-06-24" @default.
• W2220452782 creator A5004450986 @default.
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• W2220452782 creator A5049410743 @default.
• W2220452782 creator A5061321945 @default.
• W2220452782 creator A5072781436 @default.
• W2220452782 date "2016-02-01" @default.
• W2220452782 modified "2023-10-16" @default.
• W2220452782 title "Endothelial differentiation of canine yolk sac cells transduced with VEGF" @default.
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• W2220452782 doi "https://doi.org/10.1016/j.rvsc.2015.11.010" @default.
• W2220452782 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26850540" @default.
• W2220452782 hasPublicationYear "2016" @default.
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