FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2221921102> ?p ?o ?g. }

• W2221921102 endingPage "23" @default.
• W2221921102 startingPage "13" @default.
• W2221921102 abstract "Phospholipase A2 (PLA2) catalyzes the position-specific hydrolysis of fatty acids linked to the sn-2 position of phospholipids (PLs). PLA2s make up a very large superfamily, with more than known 15 groups, classified into secretory PLA2 (sPLA2), Ca2+-dependent cellular PLA2 (sPLA2) and Ca2+-independent cellular PLA2 (iPLA2). Only a few insect sPLA2s, expressed in venom glands and immune tissues, have been characterized at the molecular level. This study aimed to test our hypothesis that insects express iPLA2, using the beet armyworm, Spodoptera exigua, our model insect. Substantial PLA2 activities under calcium-free condition were recorded in several larval tissue preparations. The PLA2 activity was significantly reduced in reactions conducted in the presence of a specific iPLA2 inhibitor, bromoenol lactone (BEL). Analysis of a S. exigua hemocyte transcriptome identified a candidate iPLA2 gene (SeiPLA2-A). The open reading frame encoded 816 amino acid residues with a predicted molecular weight of 90.5 kDa and 6.15 pI value. Our phylogenetic analysis clustered SeiPLA2-A with the other vertebrate iPLA2s. SeiPLA2-A was expressed in all tissues we examined, including hemocytes, fat body, midgut, salivary glands, Malpighian tubules and epidermis. Heterologous expression in Sf9 cells indicated that SeiPLA2-A was localized in cytoplasm and exhibited significant PLA2 activity, which was independent of Ca2+ and inhibited by BEL. RNA interference (RNAi) of SeiPLA2-A using its specific dsRNA in the fifth instar larvae significantly suppressed iPLA2 expression and enzyme activity. dsSeiPLA2-A-treated larvae exhibited significant loss of cellular immune response, measured as nodule formation in response to bacterial challenge, and extended larval-to-pupal developmental time. These results support our hypothesis, showing that SeiPLA2-A predicted from the transcriptome analysis catalyzes hydrolysis of fatty acids from cellular PLs and plays crucial physiological roles in insect immunity and larval growth." @default.
• W2221921102 created "2016-06-24" @default.
• W2221921102 creator A5035215907 @default.
• W2221921102 creator A5056904924 @default.
• W2221921102 creator A5060164148 @default.
• W2221921102 creator A5065214990 @default.
• W2221921102 creator A5084504718 @default.
• W2221921102 date "2015-11-01" @default.
• W2221921102 modified "2023-10-18" @default.
• W2221921102 title "A novel calcium-independent cellular PLA2 acts in insect immunity and larval growth" @default.
• W2221921102 cites W1493442794 @default.
• W2221921102 cites W1540285469 @default.
• W2221921102 cites W1589395925 @default.
• W2221921102 cites W1964313557 @default.
• W2221921102 cites W1968982397 @default.
• W2221921102 cites W1971461328 @default.
• W2221921102 cites W1977145015 @default.
• W2221921102 cites W1988056960 @default.
• W2221921102 cites W1991024927 @default.
• W2221921102 cites W1991578658 @default.
• W2221921102 cites W1995905074 @default.
• W2221921102 cites W1997350157 @default.
• W2221921102 cites W1998849933 @default.
• W2221921102 cites W2010427635 @default.
• W2221921102 cites W2014868119 @default.
• W2221921102 cites W2015435174 @default.
• W2221921102 cites W2022202114 @default.
• W2221921102 cites W2027145809 @default.
• W2221921102 cites W2027188394 @default.
• W2221921102 cites W2027716545 @default.
• W2221921102 cites W2028690356 @default.
• W2221921102 cites W2032867124 @default.
• W2221921102 cites W2040238011 @default.
• W2221921102 cites W2042391724 @default.
• W2221921102 cites W2042415557 @default.
• W2221921102 cites W2052303476 @default.
• W2221921102 cites W2059631509 @default.
• W2221921102 cites W2066600235 @default.
• W2221921102 cites W2067421758 @default.
• W2221921102 cites W2067449259 @default.
• W2221921102 cites W2068031889 @default.
• W2221921102 cites W2068105846 @default.
• W2221921102 cites W2072263901 @default.
• W2221921102 cites W2077904086 @default.
• W2221921102 cites W2082031850 @default.
• W2221921102 cites W2083681924 @default.
• W2221921102 cites W2090045725 @default.
• W2221921102 cites W2090575626 @default.
• W2221921102 cites W2091062451 @default.
• W2221921102 cites W2091980322 @default.
• W2221921102 cites W2102678394 @default.
• W2221921102 cites W2107277218 @default.
• W2221921102 cites W2111582004 @default.
• W2221921102 cites W2111657038 @default.
• W2221921102 cites W2125702308 @default.
• W2221921102 cites W2129595368 @default.
• W2221921102 cites W2129595885 @default.
• W2221921102 cites W2154104417 @default.
• W2221921102 cites W2156747802 @default.
• W2221921102 cites W2163647890 @default.
• W2221921102 cites W2164884793 @default.
• W2221921102 cites W2168804442 @default.
• W2221921102 cites W2169554406 @default.
• W2221921102 cites W2255662319 @default.
• W2221921102 cites W2298617635 @default.
• W2221921102 cites W2407948753 @default.
• W2221921102 cites W4293247451 @default.
• W2221921102 cites W4324114017 @default.
• W2221921102 doi "https://doi.org/10.1016/j.ibmb.2015.09.012" @default.
• W2221921102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26429672" @default.
• W2221921102 hasPublicationYear "2015" @default.
• W2221921102 type Work @default.
• W2221921102 sameAs 2221921102 @default.
• W2221921102 citedByCount "45" @default.
• W2221921102 countsByYear W22219211022016 @default.
• W2221921102 countsByYear W22219211022017 @default.
• W2221921102 countsByYear W22219211022018 @default.
• W2221921102 countsByYear W22219211022019 @default.
• W2221921102 countsByYear W22219211022020 @default.
• W2221921102 countsByYear W22219211022021 @default.
• W2221921102 countsByYear W22219211022022 @default.
• W2221921102 countsByYear W22219211022023 @default.
• W2221921102 crossrefType "journal-article" @default.
• W2221921102 hasAuthorship W2221921102A5035215907 @default.
• W2221921102 hasAuthorship W2221921102A5056904924 @default.
• W2221921102 hasAuthorship W2221921102A5060164148 @default.
• W2221921102 hasAuthorship W2221921102A5065214990 @default.
• W2221921102 hasAuthorship W2221921102A5084504718 @default.
• W2221921102 hasConcept C100068826 @default.
• W2221921102 hasConcept C103878061 @default.
• W2221921102 hasConcept C104317684 @default.
• W2221921102 hasConcept C12927208 @default.
• W2221921102 hasConcept C150149183 @default.
• W2221921102 hasConcept C150194340 @default.
• W2221921102 hasConcept C153911025 @default.
• W2221921102 hasConcept C162317418 @default.
• W2221921102 hasConcept C166703698 @default.
• W2221921102 hasConcept C173758957 @default.

• next