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- W2223807384 abstract "Germ cell specification as sperm or oocyte is an ancient cell fate decision, but its molecular regulation is poorly understood. In Caenorhabditis elegans, the FOG-1 and FOG-3 proteins behave genetically as terminal regulators of sperm fate specification. Both are homologous to well-established RNA regulators, suggesting that FOG-1 and FOG-3 specify the sperm fate post-transcriptionally. We predicted that FOG-1 and FOG-3, as terminal regulators of the sperm fate, might regulate a battery of gamete-specific differentiation genes. Here we test that prediction by exploring on a genomic scale the messenger RNAs (mRNAs) associated with FOG-1 and FOG-3. Immunoprecipitation of the proteins and their associated mRNAs from spermatogenic germlines identifies 81 FOG-1 and 722 FOG-3 putative targets. Importantly, almost all FOG-1 targets are also FOG-3 targets, and these common targets are strongly biased for oogenic mRNAs. The discovery of common target mRNAs suggested that FOG-1 and FOG-3 work together. Consistent with that idea, we find that FOG-1 and FOG-3 proteins co-immunoprecipitate from both intact nematodes and mammalian tissue culture cells and that they colocalize in germ cells. Taking our results together, we propose a model in which FOG-1 and FOG-3 work in a complex to repress oogenic transcripts and thereby promote the sperm fate." @default.
- W2223807384 created "2016-06-24" @default.
- W2223807384 creator A5007506402 @default.
- W2223807384 creator A5009732614 @default.
- W2223807384 creator A5021331883 @default.
- W2223807384 creator A5029311191 @default.
- W2223807384 creator A5039540784 @default.
- W2223807384 creator A5091736792 @default.
- W2223807384 date "2015-11-09" @default.
- W2223807384 modified "2023-10-15" @default.
- W2223807384 title "Genomic Analyses of Sperm Fate Regulator Targets Reveal a Common Set of Oogenic mRNAs in <i>Caenorhabditis elegans</i>" @default.
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