FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2223880767> ?p ?o ?g. }

• W2223880767 endingPage "185" @default.
• W2223880767 startingPage "177" @default.
• W2223880767 abstract "Cells of two human follicular thyroid carcinoma cell lines (FTC133, FTC236) were stably transfected with a cDNA encoding the PDE4A cAMP-specific phosphodiesterase (PDE) splice variant RD1 (RNPDE4A1A) so as to generate the cloned cell lines, FTC133A and FTC236A. This allowed the expression of a novel rolipram-inhibited cAMP-specific PDE activity in these cells. Unlike the parent cell lines in which Ca2+/calmodulin caused a profound activation (approx. 3-4-fold) of homogenate PDE activity, no such stimulation was evident in the RD1-expressing cell lines, indicating loss of PDE1 activity. Reverse transcriptase-PCR analysis indicated that this was due to the down-regulation of the PDE1C isoform. The novel PDE4 activity in transfected cells was located exclusively in the membrane fraction, as was immunoreactive RD1. Low concentrations of the detergent Triton X-100, but not high NaCl concentrations, allowed RD1 to be solubilized. Laser scanning confocal immunofluorescence analyses identified RD1 immunoreactivity in a discrete perinuclear region of these RD1-expressing transfected cell lines. A similar pattern of labelling was observed using the antiserum Tex1, which specifically identified the Golgi apparatus. Treatment of FTC133A cells with the Golgi-perturbing agents monensin and brefeldin A led to a similar redistribution of immunoreactive species detected using both the Tex1 and anti-RD1 antisera. It is suggested that the PDE4A splice variant RD1 contains a membrane-association signal which allows the targeted expression of RD1 within the Golgi complex of these human follicular thyroid carcinoma cell lines." @default.
• W2223880767 created "2016-06-24" @default.
• W2223880767 creator A5016598709 @default.
• W2223880767 creator A5055792963 @default.
• W2223880767 creator A5058269381 @default.
• W2223880767 creator A5059630970 @default.
• W2223880767 date "1997-01-01" @default.
• W2223880767 modified "2023-09-27" @default.
• W2223880767 title "Intracellular localization of the PDE4A cAMP-specific phosphodiesterase splice variant RD1 (RNPDE4A1A) in stably transfected human thyroid carcinoma FTC cell lines" @default.
• W2223880767 cites W132750802 @default.
• W2223880767 cites W1536958645 @default.
• W2223880767 cites W1572740641 @default.
• W2223880767 cites W1776649101 @default.
• W2223880767 cites W1867807609 @default.
• W2223880767 cites W1868442457 @default.
• W2223880767 cites W1887032383 @default.
• W2223880767 cites W1887738321 @default.
• W2223880767 cites W1968133040 @default.
• W2223880767 cites W1973519389 @default.
• W2223880767 cites W1975972186 @default.
• W2223880767 cites W1990653615 @default.
• W2223880767 cites W1993584703 @default.
• W2223880767 cites W1998836600 @default.
• W2223880767 cites W2003922574 @default.
• W2223880767 cites W2007326882 @default.
• W2223880767 cites W2018289835 @default.
• W2223880767 cites W2019168667 @default.
• W2223880767 cites W2023915716 @default.
• W2223880767 cites W2028496153 @default.
• W2223880767 cites W2030636433 @default.
• W2223880767 cites W2037197202 @default.
• W2223880767 cites W2046754637 @default.
• W2223880767 cites W2047549229 @default.
• W2223880767 cites W2048497209 @default.
• W2223880767 cites W2057662508 @default.
• W2223880767 cites W2059428912 @default.
• W2223880767 cites W2064469698 @default.
• W2223880767 cites W2073654276 @default.
• W2223880767 cites W2075499154 @default.
• W2223880767 cites W2081429182 @default.
• W2223880767 cites W2084665581 @default.
• W2223880767 cites W2087149688 @default.
• W2223880767 cites W2092052750 @default.
• W2223880767 cites W2100837269 @default.
• W2223880767 cites W2111660131 @default.
• W2223880767 cites W2125255772 @default.
• W2223880767 cites W2128635872 @default.
• W2223880767 cites W2131879548 @default.
• W2223880767 cites W2147586746 @default.
• W2223880767 cites W2166389885 @default.
• W2223880767 cites W2201412268 @default.
• W2223880767 cites W2288346340 @default.
• W2223880767 cites W2301214492 @default.
• W2223880767 cites W2336257274 @default.
• W2223880767 cites W2341184974 @default.
• W2223880767 cites W2341366090 @default.
• W2223880767 cites W2418402315 @default.
• W2223880767 cites W2439507659 @default.
• W2223880767 cites W260890798 @default.
• W2223880767 cites W2949063221 @default.
• W2223880767 cites W305462020 @default.
• W2223880767 cites W4301381893 @default.
• W2223880767 doi "https://doi.org/10.1042/bj3210177" @default.
• W2223880767 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1218052" @default.
• W2223880767 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9003417" @default.
• W2223880767 hasPublicationYear "1997" @default.
• W2223880767 type Work @default.
• W2223880767 sameAs 2223880767 @default.
• W2223880767 citedByCount "35" @default.
• W2223880767 countsByYear W22238807672013 @default.
• W2223880767 countsByYear W22238807672016 @default.
• W2223880767 countsByYear W22238807672020 @default.
• W2223880767 crossrefType "journal-article" @default.
• W2223880767 hasAuthorship W2223880767A5016598709 @default.
• W2223880767 hasAuthorship W2223880767A5055792963 @default.
• W2223880767 hasAuthorship W2223880767A5058269381 @default.
• W2223880767 hasAuthorship W2223880767A5059630970 @default.
• W2223880767 hasBestOaLocation W22238807672 @default.
• W2223880767 hasConcept C104317684 @default.
• W2223880767 hasConcept C119062480 @default.
• W2223880767 hasConcept C153911025 @default.
• W2223880767 hasConcept C158617107 @default.
• W2223880767 hasConcept C187882448 @default.
• W2223880767 hasConcept C2781373781 @default.
• W2223880767 hasConcept C54009773 @default.
• W2223880767 hasConcept C54355233 @default.
• W2223880767 hasConcept C55493867 @default.
• W2223880767 hasConcept C79879829 @default.
• W2223880767 hasConcept C81885089 @default.
• W2223880767 hasConcept C86803240 @default.
• W2223880767 hasConcept C95444343 @default.
• W2223880767 hasConceptScore W2223880767C104317684 @default.
• W2223880767 hasConceptScore W2223880767C119062480 @default.
• W2223880767 hasConceptScore W2223880767C153911025 @default.
• W2223880767 hasConceptScore W2223880767C158617107 @default.
• W2223880767 hasConceptScore W2223880767C187882448 @default.
• W2223880767 hasConceptScore W2223880767C2781373781 @default.
• W2223880767 hasConceptScore W2223880767C54009773 @default.

• next